Use of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Vanillic acid (VA) found in vanilla and cinnamic acid (CA) the precursor of flavonoids and found in cinnamon oil, are natural plant phenolic acids which are secondary aromatic plant products suggested to possess many physiological and pharmacological functions. In vitro and in vivo experiments have shown that phenolic acids exhibit powerful effects on biological responses by scavenging free radicals and eliciting antioxidant capacity. In the present study, we investigated the antioxidant capacity of VA and CA by the trolox equivalent antioxidant capacity (TEAC) assay, cytotoxicity by neutral red uptake (NRU) assay in Chinese Hamster Ovary (CHO) cells and also the genotoxic and antigenotoxic effects of these phenolic acids using the cytokinesis-blocked micronucleus (CBMN) and the alkaline comet assays in human peripheral blood lymphocytes. At all tested concentrations, VA (0.17–67.2 ?g/ml) showed antioxidant activity but CA (0.15–59.2 ?g/ml) did not show antioxidant activity against 2,2-azino-bis (3-ethylbenz-thiazoline-6-sulphonic acid) (ABTS). VA (0.84, 4.2, 8.4, 16.8, 84 and 168 ?g/ml) and CA (0.74, 3.7, 7.4, 14.8, 74, 148 ?g/ml) did not have cytotoxic and genotoxic effects alone at the studied concentrations as compared with the controls. Both VA and CA seem to decrease DNA damage induced by H2O2 in human lymphocytes. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
Açıklama
Anahtar Kelimeler
CHO Cells, Cinnamic Acid, Comet Assay, Cytokinesis-Block Micronucleus Assay, Human Lymphocytes, Neutral Red Uptake Assay, Trolox Equivalent Antioxidant Capacity, Vanillic Acid
Kaynak
Drug and Chemical Toxicology
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
40
Sayı
2
Künye
Taner, G., Özkan Vardar, D., Aydin, S., Aytaç, Z., Başaran, A., & Başaran, N. (2017). Use of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid. Drug and chemical toxicology, 40(2), 183-190.
