Use of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid

Basit Öğe Kaydı
dc.authorid0000-0003-0976-9556
dc.contributor.authorTaner, Gökçe
dc.contributor.authorÖzkan Vardar, Deniz
dc.contributor.authorAydın, Sevtap
dc.contributor.authorAytaç, Zeki
dc.contributor.authorBaşaran, Ahmet
dc.contributor.authorBaşaran, Nurşen
dc.date.accessioned2019-05-10T09:39:33Z
dc.date.available2019-05-10T09:39:33Z
dc.date.issued2017
dc.departmentHitit Üniversitesi, Sungurlu Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractVanillic acid (VA) found in vanilla and cinnamic acid (CA) the precursor of flavonoids and found in cinnamon oil, are natural plant phenolic acids which are secondary aromatic plant products suggested to possess many physiological and pharmacological functions. In vitro and in vivo experiments have shown that phenolic acids exhibit powerful effects on biological responses by scavenging free radicals and eliciting antioxidant capacity. In the present study, we investigated the antioxidant capacity of VA and CA by the trolox equivalent antioxidant capacity (TEAC) assay, cytotoxicity by neutral red uptake (NRU) assay in Chinese Hamster Ovary (CHO) cells and also the genotoxic and antigenotoxic effects of these phenolic acids using the cytokinesis-blocked micronucleus (CBMN) and the alkaline comet assays in human peripheral blood lymphocytes. At all tested concentrations, VA (0.17–67.2 ?g/ml) showed antioxidant activity but CA (0.15–59.2 ?g/ml) did not show antioxidant activity against 2,2-azino-bis (3-ethylbenz-thiazoline-6-sulphonic acid) (ABTS). VA (0.84, 4.2, 8.4, 16.8, 84 and 168 ?g/ml) and CA (0.74, 3.7, 7.4, 14.8, 74, 148 ?g/ml) did not have cytotoxic and genotoxic effects alone at the studied concentrations as compared with the controls. Both VA and CA seem to decrease DNA damage induced by H2O2 in human lymphocytes. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.citationTaner, G., Özkan Vardar, D., Aydin, S., Aytaç, Z., Başaran, A., & Başaran, N. (2017). Use of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid. Drug and chemical toxicology, 40(2), 183-190.
dc.identifier.doi10.1080/01480545.2016.1190740
dc.identifier.endpage190en_US
dc.identifier.issn0148-0545
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage183en_US
dc.identifier.urihttps://doi.org/10.1080/01480545.2016.1190740
dc.identifier.urihttps://hdl.handle.net/11491/725
dc.identifier.volume40en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofDrug and Chemical Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCHO Cellsen_US
dc.subjectCinnamic Aciden_US
dc.subjectComet Assayen_US
dc.subjectCytokinesis-Block Micronucleus Assayen_US
dc.subjectHuman Lymphocytesen_US
dc.subjectNeutral Red Uptake Assayen_US
dc.subjectTrolox Equivalent Antioxidant Capacityen_US
dc.subjectVanillic Aciden_US
dc.titleUse of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu