Nucleotide incorporated magnetic microparticles for isolation of DNA

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dc.authorid0000-0003-1467-2898
dc.contributor.authorKöse, Kazım
dc.date.accessioned2019-05-13T09:03:39Z
dc.date.available2019-05-13T09:03:39Z
dc.date.issued2016
dc.departmentRektörlük
dc.description.abstractFor the comprehensive understanding of many diseases, especially genetic disease, the highly purified deoxyribonucleic acid (DNA) is needed to perform in-detail studies. There are various methods to obtain highly purified DNA, but low in number. In this study, Fe&Ni-mc-poly (2-hydroxyethyl methacrylate-adenine methacrylate) [poly(HEMA-AdeM)] microparticles were synthesized to get high purification ratio because of natural and thus selective interaction between adenine on the microparticles and thymine on the DNA molecule and also high amount of DNA purified. For the characterization of microparticles scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), elemental analysis, swelling ratio and zeta size analysis methods were performed. All experiments were performed batch-wise for the determination of optimum conditions such as pH, initial DNA concentration, temperature and interaction time. At the end of the all study, the desorption yield was obtained as 97.7% and after 5 adsorption-desorption cycle, the adsorption capacity was decreased only 3%. © 2016 Elsevier Ltd
dc.identifier.citationKöse, K. (2016). Nucleotide incorporated magnetic microparticles for isolation of DNA. Process Biochemistry, 51(10), 1644-1649.
dc.identifier.doi10.1016/j.procbio.2016.07.021
dc.identifier.endpage1649en_US
dc.identifier.issn1359-5113
dc.identifier.issue10en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage1644en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2016.07.021
dc.identifier.urihttps://hdl.handle.net/11491/1509
dc.identifier.volume51en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDNAen_US
dc.subjectDNA-Affinity Chromatographyen_US
dc.subjectMagnetic Separationen_US
dc.subjectMicroparticlesen_US
dc.subjectPeaen_US
dc.subjectSeparationen_US
dc.titleNucleotide incorporated magnetic microparticles for isolation of DNA
dc.typeArticle

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