Molecular features of follicular variant papillary carcinoma of thyroid: comparison of areas with or without classical nuclear features

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dc.contributor.authorGüney, Güven
dc.contributor.authorTezel, Gaye Güler
dc.contributor.authorKösemehmetoğlu, Kemal
dc.contributor.authorYılmaz, Engin
dc.contributor.authorBalcı, Serdar
dc.contributor.authorErsoy, Reyhan
dc.contributor.authorÇakır, Bekir
dc.contributor.authorGüler, Gülnur
dc.date.accessioned2019-05-13T08:58:53Z
dc.date.available2019-05-13T08:58:53Z
dc.date.issued2013
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü
dc.description.abstractWe aimed to compare the genetic background of different areas in follicular variant papillary thyroid carcinomas (FVPTC) with or without classical nuclear changes. Sixteen cases of FVPTC were included in our study. All tumors were well demarcated from surrounding thyroid tissue and had both areas with nuclear features (WNF) and areas without nuclear features (WONF) of papillary carcinoma. DNA is obtained by laser microdissection from WNF and WONF areas of each case. Point mutations for NRAS codon 61, HRAS codon 61, and BRAF were investigated by direct sequencing. In 11 cases, reverse transcription PCR was performed for the presence of PAX8-PPAR? and RET/PTC1–3 gene rearrangements. Point mutation for NRAS codon 61 was also studied in 15 colloidal nodules. Seven cases (44 %) showed at least one mutation; two cases (13 %) revealed the same mutation in both WNF and WONF areas, while in the rest only WNF areas were mutated. None of the studied 11 cases demonstrated RET/PTC1–3 gene rearrangement and in only one case PAX8-PPAR? gene rearrangement was found. Six cases (38 %) showed NRAS codon 61 mutation, involving only WNF areas in five cases and both WNF and WONF areas in one case. Neither HRAS codon 61 nor BRAF mutations were present. Fifteen colloidal nodules were also wild type for NRAS codon 61. Our findings suggest that NRAS codon 61 point mutations and PAX8-PPAR? gene rearrangement play a role in the FVPTC pathogenesis and may be established before the morphological/phenotypical features fully develop. © 2013, Springer Science+Business Media New York.
dc.identifier.citationGüney, G., Tezel, G. G., Kösemehmetoğlu, K., Yılmaz, E., Balcı, S., Ersoy, R., Çakır, B., Güler, G. (2014). Molecular features of follicular variant papillary carcinoma of thyroid: comparison of areas with or without classical nuclear features. Endocrine Pathology, 25(3), 241-247.
dc.identifier.doi10.1007/s12022-013-9275-6
dc.identifier.endpage247en_US
dc.identifier.issn1046-3976
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage241en_US
dc.identifier.urihttps://doi.org/10.1007/s12022-013-9275-6
dc.identifier.urihttps://hdl.handle.net/11491/1214
dc.identifier.volume25en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofEndocrine Pathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBRAFen_US
dc.subjectFollicular Variant Papillary Carcinomaen_US
dc.subjectLaser Microdissectionen_US
dc.subjectNuclear Featuresen_US
dc.subjectPAX8-PPARɣen_US
dc.subjectRASen_US
dc.subjectRET/PTCen_US
dc.subjectThyroiden_US
dc.titleMolecular features of follicular variant papillary carcinoma of thyroid: comparison of areas with or without classical nuclear features
dc.typeArticle

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