DNA adsorption via Co(II) immobilized cryogels
[ X ]
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The separation and purification of important biomolecule deoxyribonucleic acid (DNA) molecules are extremely important. The adsorption technique among these methods is highly preferred as the adsorbent cryogels are pretty much used due to large pores and the associated flow channels. In this study, the adsorption of DNA via Co(II) immobilized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [poly(HEMA-GMA)] cryogels was performed under varying conditions of pH, interaction time, initial DNA concentration, temperature, and ionic strength. For the characterization of cryogels; swelling test, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), surface area (BET), elemental and ICP-OES analysis were performed. L-lysine amino acid was chosen as Co(II)-chelating agent and the adsorption capacity of cryogels was determined as 33.81 mg DNA/g cryogel. Adsorption of pea DNA was studied under the optimum adsorption conditions and DNA adsorption capacity of cryogels was found as 10.14 mg DNA/g cryogel. The adsorption process was examined via Langmuir and Freundlich isotherm models and the Langmuir adsorption model was determined to be more appropriate for the DNA adsorption onto cryogels. © 2016 Taylor & Francis Group, LLC.
Açıklama
Anahtar Kelimeler
Adsorption, Co(II), Cryogel, DNA
Kaynak
Journal of Macromolecular Science, Part A: Pure and Applied Chemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
53
Sayı
10
Künye
Erol, K. (2016). DNA adsorption via Co (II) immobilized cryogels. Journal of Macromolecular Science, Part A, 53(10), 629-635.
