Development of a HILIC method for the determination of 5-fluorouracil from nano drug delivery systems and rat skin extracts

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dc.authorid0000-0003-2793-7154
dc.authorid0000-0001-7494-3077
dc.contributor.authorAmasya, Gülin
dc.contributor.authorGümüştaş, Mehmet
dc.contributor.authorBadıllı, Ulya
dc.contributor.authorÖzkan, Sibel Ayşıl
dc.contributor.authorTarımcı, Nilüfer
dc.date.accessioned2019-05-13T08:57:39Z
dc.date.available2019-05-13T08:57:39Z
dc.date.issued2018
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThis is the first report in literature using hydrophilic interaction liquid chromatography (HILIC) in combination with diode array detector (DAD) for stability indicating determination of 5-Fluorouracil (5-FU) from its bulk form, pharmaceutical preparations, developed solid lipid nanoparticle (SLN) and nano structured lipid carrier (NLC) drug delivery systems as well as the rat skin extracts. The separation was performed at 45 °C, on Sequant Zic HILIC (250 mm × 4.60 mm ID, 5 ?m, 200 Ao), peek HPLC column. Mobile phase is consisting of a mixture of acetonitrile: buffer containing 5 mM ammonium acetate (95:5; v/v). The pH of the mobile phase was adjusted to 7.0 using 1 M NaOH. The analysis was carried out at 0.75 mL min?1 flow rate with a detection wavelength of 265 nm and the injection volume was arranged as 10 ?L. The developed method was fully validated in accordance with the International Council on Harmonization (ICH) Guidelines. Specificity of this method was demonstrated by forced degradation studies. As a result of calibration studies, the calibration curve was found linear in the concentration range of 1–250 ?g mL-1 (R2 = 0.999). The precision of this technique calculated within the frame of intra-day and inter-day based on a percentage of relative standard deviation (RSD%) values (<2%). The limits of detection and quantification were 11 and 37 ng mL-1 respectively. On the other hand, 5-FU loaded SLN and NLC formulations with average particle size of 370 nm were also developed and compared in order to increase the permeation of drug into the rat skin. Ex-vivo Penetration/Permeation Studies indicated that higher dermal accumulation of 5-FU was obtained with NLC formulation. As a conclusion, the present work expressed the optimization and the validation of a selective, simple, precise and accurate fully validated HILIC method with sufficient sensitivity for the estimation of 5-FU in raw materials, marketed formulation and rat skin extract after applying both of the commercial product and newly developed nanoparticulate drug delivery systems on to the rat skins with high percentage recoveries. © 2018 Elsevier B.V.
dc.identifier.citationAmasya, G., Gumustas, M., Badilli, U., Ozkan, S. A., & Tarimci, N. (2018). Development of a HILIC method for the determination of 5-fluorouracil from nano drug delivery systems and rat skin extracts. Journal of pharmaceutical and biomedical analysis, 154, 285-293.
dc.identifier.doi10.1016/j.jpba.2018.03.021
dc.identifier.endpage293en_US
dc.identifier.issn0731-7085
dc.identifier.scopusqualityQ1
dc.identifier.startpage285en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2018.03.021
dc.identifier.urihttps://hdl.handle.net/11491/979
dc.identifier.volume154en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject5-Fluorouracilen_US
dc.subjectHILICen_US
dc.subjectHPLCen_US
dc.subjectNano Drug Delivery Systemen_US
dc.subjectNLCen_US
dc.subjectSLNen_US
dc.titleDevelopment of a HILIC method for the determination of 5-fluorouracil from nano drug delivery systems and rat skin extracts
dc.typeArticle

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