Role of nitric oxide synthesis inhibitors in iron-induced nigral neurotoxicity: A mechanistic exploration

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dc.contributor.authorBostancı, Mehmet Ömer
dc.contributor.authorBağırıcı, Faruk
dc.contributor.authorBaş, Orhan
dc.date.accessioned2019-05-13T09:04:30Z
dc.date.available2019-05-13T09:04:30Z
dc.date.issued2008
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractIn the central nervous system, nitric oxide (NO) has been suggested to be a cell-to-cell signaling molecule that regulates guanylyl cyclase, aconitase, and iron regulatory protein. NO is also one of the substances that is involved in neuronal death. On the other hand, iron overload and enhanced hydroxyl radical formation have been implicated as the causative factors of some neurodegenerative disorders. The present study was performed to clarify whether nitric oxide is involved in iron-induced neuron death. Neurotoxicity was produced by microinjection of iron chloride (200 mM, 2.5 ?L) into the left cerebral ventricle. After the intracerebroventricular (ICV) injection, all animals were kept alive for 10 days. During this period, animals in the iron + L-NAME (N-nitro-L-arginine methyl ester) and iron + aminoguanidine groups received intraperitoneal (IP) L-NAME (30 mg/kg) and aminoguanidine (100 mg/kg) injections once a day, respectively. Rats belonging to the control group also received intraperitoneally the same amount of saline. After 10 days, the rats were perfused intracardially under deep urethane anesthesia. Removed brains were processed using the standard histological techniques. The total numbers of neurons in substantia nigra of all rats were estimated with stereological techniques. It was found that L-NAME significantly decreased nigral cell loss from 43.2% to 14.0%, while aminoguanidine did not affect cell loss. Results of the present study suggest that NOS inhibition by L-NAME seems to have neuroprotective effects on iron-induced nigral neurotoxicity. Copyright © Informa Healthcare USA, Inc.
dc.identifier.citationBostancı, M. Ö., Bağırıcı, F., Baş, O. (2008). Role of nitric oxide synthesis inhibitors in iron-induced nigral neurotoxicity: a mechanistic exploration. Toxicology Mechanisms and Methods, 18(4), 379-384.
dc.identifier.doi10.1080/15376510801891369
dc.identifier.endpage384en_US
dc.identifier.issn1537-6516
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage379en_US
dc.identifier.urihttps://doi.org/10.1080/15376510801891369
dc.identifier.urihttps://hdl.handle.net/11491/1599
dc.identifier.volume18en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofToxicology Mechanisms and Methods
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAminoguanidineen_US
dc.subjectIronen_US
dc.subjectL-NAMEen_US
dc.subjectNeurotoxicityen_US
dc.subjectStereologyen_US
dc.subjectSubstantia Nigraen_US
dc.titleRole of nitric oxide synthesis inhibitors in iron-induced nigral neurotoxicity: A mechanistic exploration
dc.typeArticle

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