A sensitive and selective RP-LC method for the simultaneous determination of the antihypertensive drugs, enalapril, lercanidipine, nitrendipine and their validation
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Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A RP-LC method is presented, which is sensitive and selective for the simultaneous determination of enalapril-lercanidipine and enalapril-nitrendipine binary mixtures in their pharmaceutical dosage forms. The analyte peaks were detected using the LC method with the mobile phase ratio of methanol: water (70:30 v/v, pH 3.0) and a 1.0 mL min-1 flow rate. The detection wavelength was selected at 210 nm using photo diode array detector and column temperature was optimized to 30 C. Linearity was obtained at different concentration ranges for all working pharmaceutically active compounds between 0.5 and 25 ?g mL-1. The proposed methods were extensively validated according to USP 27 requirements and ICH guidelines. The methods were applied to the analysis of pharmaceutical dosage forms containing binary mixtures of enalapril-lercanidipine and enalapril-nitrendipine. Moreover, the proposed methods were applied for the degradation studies of the selected compounds. Degradation studies were conducted using stress conditions such as UV light, acidic and alkaline hydrolysis, oxidation and heat in oven, to evaluate the ability of the separation of the response of standard compounds from their degradation products. © 2013 Springer-Verlag Berlin Heidelberg.
Açıklama
Anahtar Kelimeler
Enalapril, Lercanidipine, Nitrendipine, Reversed-Phase Liquid Chromatography
Kaynak
Chromatographia
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
76
Sayı
21-22
Künye
Kurbanoglu, S., Gumustas, M., Uslu, B., Ozkan, S.A. (2013). A Sensitive and Selective RP-LC Method for the Simultaneous Determination of the Antihypertensive Drugs, Enalapril, Lercanidipine, Nitrendipine and Their Validation. Chromatographia, 76(21-22), 1477-1485.
