Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins
[ X ]
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
American Chemical Society
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
We describe a potentially universal, simple and cheap method to prepare water-compatible molecularly imprinted polymer nanoparticles (MIP-NPs) as synthetic antibodies against proteins. The strategy is based on a solid phase synthesis approach where glass beads (GBs) are functionalized with a metal chelate, acting as a general affinity ligand to attract surface-bound histidines present on proteins. This configuration enables an oriented immobilization of the proteins, upon which thermoresponsive MIP-NPs are synthesized. The GBs play the role of both a reactor and a separation column since, after synthesis, the MIP-NPs are released from the support by a simple temperature change, resulting in protein-free polymers. The resulting MIP-NPs are endowed with improved binding site homogeneity, since the binding sites have the same orientation. Moreover, they are stable (no aggregation) in a buffer solution for prolonged storage time and exhibit apparent dissociation constants in the nanomolar range, with little or no cross-reactivity toward other proteins. © 2015 American Chemical Society.
Açıklama
Anahtar Kelimeler
[Belirlenecek]
Kaynak
Biomacromolecules
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
17
Sayı
1
Künye
Xu, J., Ambrosini, S., Tamahkar, E., Rossi, C., Haupt, K., Tse Sum Bui, B. (2015). Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins. Biomacromolecules, 17(1), 345-353.
