Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins

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dc.contributor.authorXu, Jingjing
dc.contributor.authorAmbrosini, Serena
dc.contributor.authorTamahkar Irmak, Emel
dc.contributor.authorRossi, Claire
dc.contributor.authorHaupt, Karsten
dc.contributor.authorBui, Bernadette Tse Sum
dc.date.accessioned2019-05-13T09:04:29Z
dc.date.available2019-05-13T09:04:29Z
dc.date.issued2016
dc.departmentHitit Üniversitesi, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü
dc.description.abstractWe describe a potentially universal, simple and cheap method to prepare water-compatible molecularly imprinted polymer nanoparticles (MIP-NPs) as synthetic antibodies against proteins. The strategy is based on a solid phase synthesis approach where glass beads (GBs) are functionalized with a metal chelate, acting as a general affinity ligand to attract surface-bound histidines present on proteins. This configuration enables an oriented immobilization of the proteins, upon which thermoresponsive MIP-NPs are synthesized. The GBs play the role of both a reactor and a separation column since, after synthesis, the MIP-NPs are released from the support by a simple temperature change, resulting in protein-free polymers. The resulting MIP-NPs are endowed with improved binding site homogeneity, since the binding sites have the same orientation. Moreover, they are stable (no aggregation) in a buffer solution for prolonged storage time and exhibit apparent dissociation constants in the nanomolar range, with little or no cross-reactivity toward other proteins. © 2015 American Chemical Society.
dc.identifier.citationXu, J., Ambrosini, S., Tamahkar, E., Rossi, C., Haupt, K., Tse Sum Bui, B. (2015). Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins. Biomacromolecules, 17(1), 345-353.
dc.identifier.doi10.1021/acs.biomac.5b01454
dc.identifier.endpage353en_US
dc.identifier.issn1525-7797
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage345en_US
dc.identifier.urihttps://doi.org/10.1021/acs.biomac.5b01454
dc.identifier.urihttps://hdl.handle.net/11491/1597
dc.identifier.volume17en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.ispartofBiomacromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject[Belirlenecek]en_US
dc.titleToward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins
dc.typeArticle

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