Recognition of lysozyme using surface imprinted bacterial cellulose nanofibers
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Here, we developed the lysozyme imprinted bacterial cellulose (Lyz-MIP/BC) nanofibers via the surface imprinting strategy that was designed to recognize lysozyme. This study includes the molecular imprinting method onto the surface of bacterial cellulose nanofibers in the presence of lysozyme by metal ion coordination, as well as further characterizations methods FTIR, SEM and contact angle measurements. The maximum lysozyme adsorption capacity of Lyz-MIP/BC nanofibers was found to be 71 mg/g. The Lyz-MIP/BC nanofibers showed high selectivity for lysozyme towards bovine serum albumin and cytochrome c. Overall, the Lyz-MIP/BC nanofibers hold great potential for lysozyme recognition due to the high binding capacity, significant selectivity and excellent reusability. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Açıklama
Anahtar Kelimeler
Bacterial Cellulose Nanofibers, Lysozyme Recognition, Metal Ion Coordination, Protein Purification, Surface Imprinting
Kaynak
Journal of Biomaterials Science, Polymer Edition
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
28
Sayı
16
Künye
Saylan, Y., Tamahkar, E., Denizli, A. (2017). Recognition of lysozyme using surface imprinted bacterial cellulose nanofibers. Journal of Biomaterials Science, Polymer Edition, 28(16), 1950-1965.
