Effects of silver sulfide quantum dots coated with 2-mercaptopropionic acid on genotoxic and apoptotic pathways in vitro

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dc.contributor.authorÖzkan Vardar, Deniz
dc.contributor.authorAydın, Sevtap
dc.contributor.authorHocaoğlu, İbrahim
dc.contributor.authorYağcı Acar, Funda Havva
dc.contributor.authorBaşaran, Nursen
dc.date.accessioned2019-05-10T09:38:49Z
dc.date.available2019-05-10T09:38:49Z
dc.date.issued2018
dc.departmentHitit Üniversitesi, Sungurlu Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractQuantum dots (QDs) are highly promising nanomaterials in bioimaging system because of their bright fluorescence, broad UV excitation, narrow emission band, and high photostability. Recently, there is a great activity on Ag2S quantum dots for both imaging and drug/gene delivery due to the potential of having a better cytocompatability and near infrared luminescence. 2-Mercaptopropionic acid (2 MP A)-coated silver sulfide (Ag2S) QDs were reported as the most luminescent, stable, anionic Ag2S QDs in the literature. In this study, we aim to determine the cytotoxicity of 2 MP A/Ag2S in Chinese hamster lung fibroblast (V79) cells. The genotoxic and apoptotic effects of 2 MP A/Ag2S QDs were assessed by the alkaline single cell electrophoresis assay and real time polymerase chain reaction techniques, respectively. The cell viability decreased above 200 ?g/ml and 800 ?g/ml for MTT tetrazolium and neural red uptake assays, respectively. DNA damage was not observed by 2 MP A/Ag2S QDs at the studied concentration levels (5–2000 ?g/ml). The levels of mRNA expression of p53, caspase 3, caspase 9, bax, bcl-2, survivin were not changed by 2 MP A/Ag2S QDs below IC50 (around 1000 ?g/ml). Hence, 2 MP A/Ag2S QDs did not show any cytotoxic or genotoxic effects in V79 cells at lower doses. We conclude that the biocompatibility of 2 MP A/Ag2SODs makes them suitable for cell labeling applications. © 2018 Elsevier B.V.
dc.identifier.citationVardar, D.O., Aydin, S., Hocaoglu, I., Acar, F.H., Başaran, N. (2018). Effects of silver sulfide quantum dots coated with 2-mercaptopropionic acid on genotoxic and apoptotic pathways in vitro. Chemico-biological interactions, 291, 212-219 .
dc.identifier.doi10.1016/j.cbi.2018.06.032
dc.identifier.endpage219en_US
dc.identifier.issn0009-2797
dc.identifier.scopusqualityQ1
dc.identifier.startpage212en_US
dc.identifier.urihttps://doi.org/10.1016/j.cbi.2018.06.032
dc.identifier.urihttps://hdl.handle.net/11491/491
dc.identifier.volume291en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofChemico-Biological Interactions
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject2-Mercaptopropionic Acid-Coated Silver Sulfide Quantum Dotsen_US
dc.subjectApoptosisen_US
dc.subjectCytotoxicityen_US
dc.subjectGenotoxicityen_US
dc.subjectReal Time Polymerase Chain Reactionen_US
dc.titleEffects of silver sulfide quantum dots coated with 2-mercaptopropionic acid on genotoxic and apoptotic pathways in vitro
dc.typeArticle

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