Simultaneous determination and validation of emtricitabine, rilpivirine and tenofovir from biological samples using LC and CE methods

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dc.contributor.authorGümüştaş, Mehmet
dc.contributor.authorÇağlayan, Mehmet Gökhan
dc.contributor.authorOnur, Feyyaz
dc.contributor.authorÖzkan, Sibel Ayşıl
dc.date.accessioned2019-05-10T09:39:54Z
dc.date.available2019-05-10T09:39:54Z
dc.date.issued2018
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractA combination of antiretroviral agents is frequently used in effective treatment of the human immunodeficiency virus infection. In this study, two different separation methods are presented for the simultaneous determination of emtricitabine, rilpivirine and tenofovir from raw materials and urine samples. Developed liquid chromatography and capillary electrophoresis methods were thoroughly optimized for high analytical performances. Optimization of multiple variables at the same time by performing a minimum number of experiments was achieved by the Box–Behnken design, which is an experimental design in response surface methodology, in capillary electrophoresis. The results of the experimental design ensure minimum analysis time with well-separated analytes. Separation conditions, such as different stationary phases, pH level, organic modifiers and temperatures in liquid chromatography method, were also optimized. In particular, among stationary phases, the core–shell column especially enhanced the effectiveness of separation in liquid chromatography. Both methods were fully validated and applied to real samples. The main advantage of the developed methods is the separation of the drug combination in a short time with high efficiency and without any time-consuming steps. Copyright © 2017 John Wiley & Sons, Ltd.
dc.identifier.citationGümüştaş, M., Çağlayan, M. G., Onur, F., Özkan, S. A. (2018). Simultaneous determination and validation of emtricitabine, rilpivirine and tenofovir from biological samples using LC and CE methods. Biomedical Chromatography, 32(4), e4158.
dc.identifier.doi10.1002/bmc.4158
dc.identifier.issn0269-3879
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1002/bmc.4158
dc.identifier.urihttps://hdl.handle.net/11491/803
dc.identifier.volume32en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherJohn Wiley and Sons Ltd
dc.relation.ispartofBiomedical Chromatography
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiologicalen_US
dc.subjectCapillary Electrophoresisen_US
dc.subjectEmtricitabineen_US
dc.subjectHPLCen_US
dc.subjectRilpivirineen_US
dc.subjectTenofoviren_US
dc.subjectUrineen_US
dc.titleSimultaneous determination and validation of emtricitabine, rilpivirine and tenofovir from biological samples using LC and CE methods
dc.typeArticle

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