Effect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels

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dc.authorscopusid56589673400
dc.authorscopusid57204707220
dc.authorscopusid55251840900
dc.authorscopusid8870164800
dc.contributor.authorErol, Kadir
dc.contributor.authorKoncuk Cebeci, Büşra
dc.contributor.authorKöse, Kazım
dc.contributor.authorKöse, Dursun Ali
dc.date.accessioned2021-11-01T14:51:55Z
dc.date.available2021-11-01T14:51:55Z
dc.date.issued2019
dc.departmentHitit Üniversitesi, Alaca Avni Çelik Meslek Yüksekokulu, Gıda İşleme Bölümü
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.departmentHitit Üniversitesi, Osmancık Ömer Derindere Meslek Yüksekokulu, Mülkiyet Koruma ve Güvenlik Bölümü
dc.description.abstractHydrogen peroxide is converted by catalase to molecular oxygen and water to remove oxidative stress. In this study, catalase immobilization was performed using poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) (poly(HEMA-GMA)) cryogels with different amounts of GMA. Catalase adsorption capacity of 298.7 ± 9.9 mg/g was achieved at the end of 9 h using the poly(HEMA-GMA)-250 cryogel. Kinetic parameters and the inhibitory effects of pesticides such as 4,4?-DDE and 4,4?-DDT on the activity of free and immobilized catalase enzyme were investigated. While the V max value of the immobilized enzyme was reduced 4-fold compared to the free enzyme, in the case of the comparison of the K M values, the affinity of the immobilized enzyme was increased by 1.94 times against the substrate. The inhibitory effect of 4,4?-DDT pesticide was found to be higher for the immobilized and free enzyme. NaCl (1 M, pH: 7.0) solution was used for desorption of the adsorbed catalase enzyme. A desorption ratio of 96.45% was achieved. The technique used in this study is promising regarding for the immobilization of catalase enzyme to increase the operational activity. Therefore, poly(HEMA-GMA) cryogels have the potential to be used for immobilization of catalase enzyme in the fields of biology and biochemistry. © 2018 Elsevier B.V.
dc.description.sponsorshipFirat University Scientific Research Projects Management Unit, FÃœBAP: ODMYO19001.17.002en_US
dc.description.sponsorshipThis work was supported by the Hitit University Scientific Research Projects Coordination Unit [grant numbers ODMYO19001.17.002 ].en_US
dc.description.sponsorshipThe authors of this research, which was supported by the Hitit University Scientific Research Projects Coordination Unit with a grant number as ODMYO19001.17.002 , was hugely indebted to the Hitit University.en_US
dc.identifier.citationErol, K., Cebeci, B. K., Köse, K., & Köse, D. A. (2019). Effect of immobilization on the activity of catalase carried by poly (HEMA-GMA) cryogels. International journal of biological macromolecules, 123, 738-743.
dc.identifier.doi10.1016/j.ijbiomac.2018.11.121
dc.identifier.endpage743en_US
dc.identifier.issn0141-8130
dc.identifier.pmid30452980
dc.identifier.scopus2-s2.0-85056775135
dc.identifier.scopusqualityQ1
dc.identifier.startpage738en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2018.11.121
dc.identifier.urihttps://hdl.handle.net/11491/6379
dc.identifier.volume123en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorErol, Kadir
dc.institutionauthorKoncuk Cebeci, Büşra
dc.institutionauthorKöse, Kazım
dc.institutionauthorKöse, Dursun Ali
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCatalaseen_US
dc.subjectImmobilizationen_US
dc.subjectOperational Activityen_US
dc.titleEffect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels
dc.typeArticle

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