Investigation of heme oxygenase 2 enzyme protein expression in keratoconus and normal human corneal epithelium: an immunohistochemical study

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dc.contributor.authorÇağlayan, Mehtap
dc.contributor.authorKocamış, Sücattin İlker
dc.contributor.authorSaraç, Özge
dc.contributor.authorTatlı Doğan, Hayriye
dc.contributor.authorKösekahya, Pınar
dc.contributor.authorAyan, Murat
dc.contributor.authorÇağıl, Nurullah
dc.date.accessioned2019-05-10T09:39:54Z
dc.date.available2019-05-10T09:39:54Z
dc.date.issued2019
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü
dc.description.abstractPurpose: To compare heme oxygenase 2 (HO-2) enzyme levels detected by immunohistochemical staining methods in the cornea epithelium obtained from keratoconus patients and normal subjects. Materials and Methods: The keratoconus group included 69 eyes of 69 patients with keratoconus scheduled for cross-linking surgery. The control group included 52 eyes of 52 patients with refractive error scheduled for photorefractive keratectomy surgery. After a detailed ophthalmologic examination, corneal topographic maps of each patient were generated, and then the patients underwent surgery. The corneal epithelium was collected mechanically during the surgery, fixed with formalin, embedded in paraffin blocks, and sectioned by microtomes. HO-2 antibodies were applied to the samples for immunohistochemical evaluation. The intensity of the staining was identified as negative, weak, moderate or strong. The keratoconus group was classified as early (average keratometry (AvrK) ? 47 D), moderate (AvrK 47–55 D) and advanced keratoconus (AvrK ? 55 D). Finally, intergroup and intragroup comparison analyses were made statistically. Results: In the keratoconus group, 20 (29%) (14 weak and 6 moderate staining) of the 69 corneal epithelial specimens were identified with HO-2 expression. In the control group, 40 (76.9%) (16 moderate and 24 strong staining) of the 52 corneal epithelial specimens were identified with HO-2 expression. HO-2 expression in the corneal epithelial specimens was significantly less in the keratoconus group than in the control group (p < 0.001). There was no substantial difference among the keratoconus subgroups in terms of staining with the HO-2 antibody (p = 0.797). Conclusions: The HO-2 enzyme staining using immunohistochemical methods was at lower amounts in the keratoconic corneal epithelial cells as compared with normal corneal epithelial cells. The HO-2 enzyme may play a role in the etiopathogenesis of keratoconus. © 2018, © 2018 Taylor & Francis Group, LLC.
dc.identifier.citationÇağlayan, M., Kocamış, S. İ., Saraç, O., Tatlı Doğan, H., Kösekahya, P., Ayan, M., Çağıl, N. (2019). Investigation of heme oxygenase 2 enzyme protein expression in keratoconus and normal human corneal epithelium: an immunohistochemical study. Current Eye Research, 44(1), 25-29.
dc.identifier.doi10.1080/02713683.2018.1521980
dc.identifier.endpage29en_US
dc.identifier.issn0271-3683
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage25en_US
dc.identifier.urihttps://doi.org/10.1080/02713683.2018.1521980
dc.identifier.urihttps://hdl.handle.net/11491/804
dc.identifier.volume44en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofCurrent Eye Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCorneal Epitheliumen_US
dc.subjectHeme Oxygenase-2en_US
dc.subjectImmunohistochemistryen_US
dc.subjectKeratoconusen_US
dc.subjectOxidative Stressen_US
dc.titleInvestigation of heme oxygenase 2 enzyme protein expression in keratoconus and normal human corneal epithelium: an immunohistochemical study
dc.typeArticle

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