Lectin histochemistry of Agrotis segetum midgut cells and peritrophic membrane
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Gazi University
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Midgut cell and peritrophic membranes of grain pest Agrotis segetum larvae were examined by light microscope. Six biotinylated lectins were used in the assays by applying histochemical methods (Avidin-Biotin-Peroxidase). The aim of this study was to find lectins binding on peritrophic membranes if they were available as insecticidal agents. Results of the study indicated that lectins BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia) strongly stained whereas, PNA (Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stained the tissues. However, WGA (Wheat Germ Agglutinin) and Con-A (Canavalia ensiformis) stained the tissues weakly. The common feature of two binding proteins, BPA and GS-I lectin was binding to D-galactose. Our examinations revealed that D-galactose mostly exists in A. segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GS-I lectins can be used as insecticidal agents against A. segetum. © 2018, Gazi University Eti Mahallesi. All rights reserved.
Açıklama
Anahtar Kelimeler
Agrotis Segetum Larvae, Biotinylated Lectins, Midgut Cells, Peritrophic Membrane
Kaynak
Gazi University Journal of Science
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
31
Sayı
4
Künye
Shomali-Moghaddam, N., Gül, N., Gökalp Özkorkmaz, E., Özlük, A. (2018). Lectin histochemistry of Agrotis segetum midgut cells and peritrophic membrane. Gazi University Journal of Science, 31(4), 1022-1031.
