Development and validation of a liquid chromatographic method for concurrent assay of weakly basic drug verapamil and amphoteric drug trandolapril in pharmaceutical formulations
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Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The analysis of weakly basic drugs such as verapamil by reverse-phase liquid chromatography remains a problem, particularly when present in combination with other drugs such as amphoteric compounds like trandolapril. In this study, the simple, accurate, precise and fully validated RP-LC method for the simultaneous determination of verapamil and trandolapril in combined dosage forms has been developed. The LC method allowed quantitation over the ranges of 0.50-18.00 ?g/mL and 0.05-1.00 ?g/mL for verapamil and trandolapril, respectively. The detection limits were found to be 0.008 ?g/mL and 0.018 ?g/mL for verapamil and trandolapril, respectively. Moreover, pKa values of verapamil and trandolapril were determined via the dependence of the retention factor on the pH of the mobile phase for ionizable substances. The effect of the mobile phase composition on the ionization constant was studied by measuring the pKa at different methanol-water mixtures, ranging 50-65% (v/v). It was shown that RP-HPLC was suitable for the high throughput analysis of the combination of verapamil and trandolapril. The method also allows a number of cost and time saving benefits and can be readily employed for the analysis of pharmaceutical formulations. The method has been verified, without any interference from excipients, for the concurrent analysis of these compounds in tablets.
Açıklama
Anahtar Kelimeler
HPLC, PK A, Ramipril, Simultaneous Determination, Trandolapril, Verapamil
Kaynak
Journal of Food and Drug Analysis
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
20
Sayı
3
Künye
Gumustas, M., Sanli, S., Sanli, N., & OzkAN, S. A. (2012). Development and Validation of a Liquid Chromatographic Method for Concurrent Assay of Weakly Basic Drug Verapamil and Amphoteric Drug Trandolapril in Pharmaceutical Formulations. Journal of Food & Drug Analysis, 20(3).
