Development and validation of a liquid chromatographic method for concurrent assay of weakly basic drug verapamil and amphoteric drug trandolapril in pharmaceutical formulations

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dc.contributor.authorGümüştaş, Mehmet
dc.contributor.authorŞanlı, Senem
dc.contributor.authorŞanlı, Nurullah
dc.contributor.authorÖzkan, Sibel Ayşıl
dc.date.accessioned2019-05-13T08:58:47Z
dc.date.available2019-05-13T08:58:47Z
dc.date.issued2012
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThe analysis of weakly basic drugs such as verapamil by reverse-phase liquid chromatography remains a problem, particularly when present in combination with other drugs such as amphoteric compounds like trandolapril. In this study, the simple, accurate, precise and fully validated RP-LC method for the simultaneous determination of verapamil and trandolapril in combined dosage forms has been developed. The LC method allowed quantitation over the ranges of 0.50-18.00 ?g/mL and 0.05-1.00 ?g/mL for verapamil and trandolapril, respectively. The detection limits were found to be 0.008 ?g/mL and 0.018 ?g/mL for verapamil and trandolapril, respectively. Moreover, pKa values of verapamil and trandolapril were determined via the dependence of the retention factor on the pH of the mobile phase for ionizable substances. The effect of the mobile phase composition on the ionization constant was studied by measuring the pKa at different methanol-water mixtures, ranging 50-65% (v/v). It was shown that RP-HPLC was suitable for the high throughput analysis of the combination of verapamil and trandolapril. The method also allows a number of cost and time saving benefits and can be readily employed for the analysis of pharmaceutical formulations. The method has been verified, without any interference from excipients, for the concurrent analysis of these compounds in tablets.
dc.identifier.citationGumustas, M., Sanli, S., Sanli, N., & OzkAN, S. A. (2012). Development and Validation of a Liquid Chromatographic Method for Concurrent Assay of Weakly Basic Drug Verapamil and Amphoteric Drug Trandolapril in Pharmaceutical Formulations. Journal of Food & Drug Analysis, 20(3).
dc.identifier.doi10.6227/jfda.2012200304
dc.identifier.endpage596+713en_US
dc.identifier.issn1021-9498
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage588en_US
dc.identifier.urihttps://doi.org/10.6227/jfda.2012200304
dc.identifier.urihttps://hdl.handle.net/11491/1198
dc.identifier.volume20en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofJournal of Food and Drug Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHPLCen_US
dc.subjectPK Aen_US
dc.subjectRamiprilen_US
dc.subjectSimultaneous Determinationen_US
dc.subjectTrandolaprilen_US
dc.subjectVerapamilen_US
dc.titleDevelopment and validation of a liquid chromatographic method for concurrent assay of weakly basic drug verapamil and amphoteric drug trandolapril in pharmaceutical formulations
dc.typeArticle

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