Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization

dc.authoridTatar, Demet / 0000-0002-9317-3263
dc.authoridVeyisoğlu, Aysel / 0000-0002-1406-5513
dc.authoridErol, Kadir / 0000-0001-9158-6091
dc.authorwosidTatar, Demet / AAY-4347-2021
dc.authorwosidTatar, Demet / AAY-4486-2021
dc.authorwosidVeyisoglu, Aysel / AAY-9698-2021
dc.contributor.authorErol, Kadir
dc.contributor.authorTatar, Demet
dc.contributor.authorVeyisoğlu, Aysel
dc.contributor.authorTokatlı, Ali
dc.date.accessioned2021-11-01T15:05:54Z
dc.date.available2021-11-01T15:05:54Z
dc.date.issued2021
dc.departmentHitit Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractMicron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.
dc.identifier.citationErol, K., Tatar, D., Veyisoğlu, A., & Tokatlı, A. (2021). Antimicrobial magnetic poly (GMA) microparticles: synthesis, characterization and lysozyme immobilization. Journal of Polymer Engineering, 41(2), 144-154.
dc.identifier.doi10.1515/polyeng-2020-0191
dc.identifier.endpage154en_US
dc.identifier.issn0334-6447
dc.identifier.issn2191-0340
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85098848409
dc.identifier.scopusqualityQ3
dc.identifier.startpage144en_US
dc.identifier.urihttps://doi.org/10.1515/polyeng-2020-0191
dc.identifier.urihttps://hdl.handle.net/11491/7436
dc.identifier.volume41en_US
dc.identifier.wosWOS:000626330500007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorErol, Kadir
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofJournal Of Polymer Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectActivityen_US
dc.subjectAdsorptionen_US
dc.subjectEnzyme Immobilizationen_US
dc.subjectlysozymeen_US
dc.subjectMagnetic Microparticleen_US
dc.titleAntimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
dc.typeArticle

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