A comparative study on the in vitro cytotoxic responses of two mammalian cell types to fullerenes, carbon nanotubes and iron oxide nanoparticles

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dc.authorid0000-0003-3668-6975
dc.contributor.authorDönmez Güngüneş, Çiğdem
dc.contributor.authorŞeker, Şükran
dc.contributor.authorEser Elçin, Ayşe
dc.contributor.authorElçin, Yaşar Murat
dc.date.accessioned2019-05-10T09:39:33Z
dc.date.available2019-05-10T09:39:33Z
dc.date.issued2017
dc.departmentHitit Üniversitesi, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü
dc.description.abstractThe present study was designed to evaluate and compare the time- and dose-dependent cellular response of human periodontal ligament fibroblasts (hPDLFs), and mouse dermal fibroblasts (mDFs) to three different types of nanoparticles (NPs); fullerenes (C60), single walled carbon nanotubes (SWCNTs) and iron (II,III) oxide (Fe3O4) nanoparticles via in vitro toxicity methods, and impedance based biosensor system. NPs were characterized according to their morphology, structure, surface area, particle size distribution and zeta potential by using transmission electron microscopy, X-ray diffraction, Brunauer–Emmett–Teller, dynamic light scattering and zeta sizer analyses. The Mössbauer spectroscopy was used in order to magnetically characterize the Fe3O4 NPs. The hPDLFs and mDFs were exposed to different concentrations of the NPs (0.1, 1, 10, 50 and 100 ?g g/mL) for predetermined time intervals (6, 24 and 48 h) under controlled conditions. Subsequently, NP exposed cells were tested for viability, membrane leakage and generation of intracellular reactive oxygen species. Additional to in vitro cytotoxicity assays, the cellular responses to selected NPs were determined in real time using an impedance based biosensor system. Taken together, information obtained from all experiments suggests that toxicity of the selected NPs is cell type, concentration and time dependent. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.citationDönmez Güngüneş, Ç., Şeker, Ş., Elçin, A. E., & Elçin, Y. M. (2017). A comparative study on the in vitro cytotoxic responses of two mammalian cell types to fullerenes, carbon nanotubes and iron oxide nanoparticles. Drug and chemical toxicology, 40(2), 215-227.
dc.identifier.doi10.1080/01480545.2016.1199563
dc.identifier.endpage227en_US
dc.identifier.issn0148-0545
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage215en_US
dc.identifier.urihttps://doi.org/10.1080/01480545.2016.1199563
dc.identifier.urihttps://hdl.handle.net/11491/724
dc.identifier.volume40en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofDrug and Chemical Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCarbon Nanotubeen_US
dc.subjectCytotoxicityen_US
dc.subjectFullereneen_US
dc.subjectImpedance Based Assayen_US
dc.subjectIn Vitro Toxicityen_US
dc.subjectIron (II,III) Oxideen_US
dc.subjectNanoparticlesen_US
dc.titleA comparative study on the in vitro cytotoxic responses of two mammalian cell types to fullerenes, carbon nanotubes and iron oxide nanoparticles
dc.typeArticle

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