Effects of EF-24, RAD001, and paclitaxel on the expression profiles of apoptotic and anti-apoptotic genes

Basit Öğe Kaydı
dc.authorid0000-0002-4368-0777
dc.contributor.authorAlp, Ebru
dc.contributor.authorYılmaz, Akın
dc.contributor.authorÖnen, İlke Hacer
dc.contributor.authorMenevşe, Emine Sevda
dc.date.accessioned2019-05-13T08:58:35Z
dc.date.available2019-05-13T08:58:35Z
dc.date.issued2017
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractContext: Cancer cells exert differential responses to chemotherapeutics and inhibitors. To the best of our knowledge, a few or no research has been performed until now to determine the effect of EF-24 and RAD001 on MDA-MB-231 breast cancer cells with regard to mRNA expression of apoptotic and anti-apoptotic genes. Aims: In this study, we aimed to investigate the mRNA expression levels of apoptotic (caspase 2 [CASP2], CASP8, and CASP9) and anti-apoptotic (B-cell lymphoma 2 [BCL2] and BCL2-like protein 1 [BCL2L1]) genes after exposure to paclitaxel, EF-24, and RAD001 in MDA-MB-231 cells. Materials and Methods: After treatment, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to measure cell viability. mRNA expressions were analyzed using quantitative real-time polymerase chain reaction. Results: Decrease in cell viability ratios was seen in a dose-dependent manner for all chemicals. MDA-MB-231 cells responded slightly different to paclitaxel, EF-24, and RAD001 at the transcriptional level of apoptotic and anti-apoptotic genes. Conclusions: Our results showed that response of these cells to paclitaxel, EF-24, and RAD001 was found different at the transcriptional level of apoptotic and antiapoptotic genes. Therefore, understanding transcriptional changes after these drug exposure may give us a change to figure out more realistic results of the apoptotic pathway inhibition. © 2017 Indian Journal of Ophthalmology | Published by Wolters Kluwer - Medknow.
dc.identifier.citationAlp, E., Yılmaz, A., Önen, H. İ., Menevşe, E. S. (2017). Effects of EF-24, RAD001, and paclitaxel on the expression profiles of apoptotic and anti-apoptotic genes. Journal of Cancer Research and Therapeutics, 13(2), 346-350.
dc.identifier.doi10.4103/0973-1482.176172
dc.identifier.endpage350en_US
dc.identifier.issn0973-1482
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage346en_US
dc.identifier.urihttps://doi.org/10.4103/0973-1482.176172
dc.identifier.urihttps://hdl.handle.net/11491/1163
dc.identifier.volume13en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWolters Kluwer - Medknow
dc.relation.ispartofJournal of Cancer Research and Therapeutics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/3.0/*
dc.subjectApoptosisen_US
dc.subjectBreast Canceren_US
dc.subjectEF-24en_US
dc.subjectPaclitaxelen_US
dc.subjectRAD001en_US
dc.titleEffects of EF-24, RAD001, and paclitaxel on the expression profiles of apoptotic and anti-apoptotic genes
dc.typeArticle

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