Affinity binding of proteins to the modified bacterial cellulose nanofibers
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The potential of the modified bacterial cellulose (BC) nanofibers was determined bearing metal ion coordination interactions to enhance the protein adsorption and binding capacity. Thus, a household synthesized metal chelating monomer, namely N-methacryloyl-L-histidine methylester (MAH), and a commercial metal chelating monomer, namely 4-vinylimidazole (VIm), were used to complex with metal ions Cu(II) and Ni(II) respectively for the synthesis of the modified BC nanofibers. The modified nanofibers were characterized by FT-IR, SEM and EDX measurements. The protein adsorption tests were carried out using hemoglobin as a model protein and it was determined that the maximum adsorption capacity of hemoglobin onto the modified BC nanofibers was found as 47.40 mg/g. The novel strategy for the preparation of metal chelated nanofibers was developed. © 2017 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Bacterial Cellulose Nanofibers, Metal Ion Coordination, Protein Recognition
Kaynak
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
1052
Sayı
Künye
Bakhshpour, M., Tamahkar, E., Andaç, M., Denizli, A. (2017). Affinity binding of proteins to the modified bacterial cellulose nanofibers. Journal of Chromatography B, 1052, 121-127.
