Affinity binding of proteins to the modified bacterial cellulose nanofibers

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dc.contributor.authorBakhshpour, Monireh
dc.contributor.authorTamahkar Irmak, Emel
dc.contributor.authorAndaç, Müge
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-05-13T09:04:49Z
dc.date.available2019-05-13T09:04:49Z
dc.date.issued2017
dc.departmentHitit Üniversitesi, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü
dc.description.abstractThe potential of the modified bacterial cellulose (BC) nanofibers was determined bearing metal ion coordination interactions to enhance the protein adsorption and binding capacity. Thus, a household synthesized metal chelating monomer, namely N-methacryloyl-L-histidine methylester (MAH), and a commercial metal chelating monomer, namely 4-vinylimidazole (VIm), were used to complex with metal ions Cu(II) and Ni(II) respectively for the synthesis of the modified BC nanofibers. The modified nanofibers were characterized by FT-IR, SEM and EDX measurements. The protein adsorption tests were carried out using hemoglobin as a model protein and it was determined that the maximum adsorption capacity of hemoglobin onto the modified BC nanofibers was found as 47.40 mg/g. The novel strategy for the preparation of metal chelated nanofibers was developed. © 2017 Elsevier B.V.
dc.identifier.citationBakhshpour, M., Tamahkar, E., Andaç, M., Denizli, A. (2017). Affinity binding of proteins to the modified bacterial cellulose nanofibers. Journal of Chromatography B, 1052, 121-127.
dc.identifier.doi10.1016/j.jchromb.2017.03.021
dc.identifier.endpage127en_US
dc.identifier.issn1570-0232
dc.identifier.scopusqualityQ2
dc.identifier.startpage121en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2017.03.021
dc.identifier.urihttps://hdl.handle.net/11491/1647
dc.identifier.volume1052en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBacterial Cellulose Nanofibersen_US
dc.subjectMetal Ion Coordinationen_US
dc.subjectProtein Recognitionen_US
dc.titleAffinity binding of proteins to the modified bacterial cellulose nanofibers
dc.typeArticle

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