Reducing lactose content of milk from livestock and humans via lactose imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid) cryogels

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dc.contributor.authorErol, Kadir
dc.contributor.authorArslan Akveran, Gönül
dc.contributor.authorKöse, Kazım
dc.contributor.authorKöse, Dursun Ali
dc.date.accessioned2021-11-01T15:05:24Z
dc.date.available2021-11-01T15:05:24Z
dc.date.issued2021
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.departmentHitit Üniversitesi, Rektörlük
dc.departmentHitit Üniversitesi, Alaca Avni Çelik Meslek Yüksekokulu, Mülkiyet Koruma ve Güvenlik Bölümü
dc.description.abstractLactase, which can cause lactose intolerance in its deficiency, is a vital enzyme concerning digestion. To overcome lactose intolerance for patients with digestion problem depending of this kind of issue, lactose in food should be removed. In this study, lactose imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid), poly(HEMA-MAsp), cryogels were synthesized to reduce the amount of lactose content of milk samples. Occurrence of desired bounds, structural integrity, and surface characteristics were analyzed via Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), scanning electron microscope (SEM), micro computed tomography (CT), and confocal microscope methods. Water retention characteristic were tested in solution with different electrolytic nature. Adsorption parameters were optimized in an aqueous medium. The adsorption performance of imprinted cryogels was studied in milk samples obtained from cow, sheep, goat, buffalo, and from human volunteers at different intervals after birth. Amount of lactose adsorbed in aqueous media and milk sample from humans were 322 (56.7%) and 179.5 (5.94%) mg lactose/g polymer, respectively. Selectivity studies revealed an approximately 8-fold increase in adsorption rate of molecularly imprinted cryogels as compared to that of nonimprinted cryogels. In addition, competitive adsorption was conducted using lactose-imprinted cryogels in aqueous media containing lactose, glucose, and galactose molecules resulting in adsorption rates of 220.56, 57.87, and 61.65 mg biomolecule/g polymer, respectively.
dc.description.sponsorshipHitit University Scientific Research Projects Coordination UnitHitit University [ALACA19001.17.001]en_US
dc.description.sponsorshipThis study was supported by Hitit University Scientific Research Projects Coordination Unit with the project ALACA19001.17.001.en_US
dc.identifier.citationErol, K., Akveran, G. A., Köse, K., & Köse, D. A. (2021). Reducing lactose content of milk from livestock and humans via lactose imprinted poly (2-hydroxyethyl methacrylate-N-methacryloyl-i-aspartic acid) cryogels. Journal of Polymer Engineering.
dc.identifier.doi10.1515/polyeng-2020-0176
dc.identifier.endpage596en_US
dc.identifier.issn0334-6447
dc.identifier.issn2191-0340
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-85108100835
dc.identifier.scopusqualityQ3
dc.identifier.startpage585en_US
dc.identifier.urihttps://doi.org/10.1515/polyeng-2020-0176
dc.identifier.urihttps://hdl.handle.net/11491/7258
dc.identifier.volume41en_US
dc.identifier.wosWOS:000681143500006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorErol, Kadir
dc.institutionauthorKöse, Kazım
dc.institutionauthorKöse, Dursun Ali
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofJournal Of Polymer Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryogelsen_US
dc.subjectLactose Intoleranceen_US
dc.subjectMilken_US
dc.subjectMolecular Imprintingen_US
dc.subjectRemovalen_US
dc.titleReducing lactose content of milk from livestock and humans via lactose imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid) cryogels
dc.typeArticle

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