Polyethyleneimine assisted-two-step polymerization to develop surface imprinted cryogels for lysozyme purification

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dc.contributor.authorErol, Kadir
dc.contributor.authorKöse, Kazım
dc.contributor.authorUzun, Lokman
dc.contributor.authorSay, Rıdvan
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-05-13T08:58:08Z
dc.date.available2019-05-13T08:58:08Z
dc.date.issued2016
dc.departmentHitit Üniversitesi, Alaca Avni Çelik Meslek Yüksekokulu, Mülkiyet Koruma ve Güvenlik Bölümü
dc.description.abstractSurface imprinting strategy is one of the promising approaches to synthesize plastic antibodies while overcoming the problems in the protein imprinting research. In this study, we focused our attentions on developing two-step polymerization to imprint on the bare surface employing polyethyleneimine (PEI) assisted-coordination of template molecules, lysozyme. For this aim, we firstly synthesized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), poly(HEMA-GMA) cryogels as a bare structure. Then, we immobilized PEI onto the cryogels through the addition reaction between GMA and PEI molecules. After that, we determined the amount of free amine (NH2) groups of PEI molecules, subsequently immobilized methacrylate functionalities onto the half of them and another half was used to chelate Cu(II) ions as a mediator between template, lysozyme and PEI groups. After the characterization of the materials developed by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and the micro-computed tomography (?CT), we optimized the lysozyme adsorption conditions from aqueous solution. Before performing lysozyme purification from chicken egg white, we evaluated the effects of pH, interaction time, the initial lysozyme concentration, temperature and ionic strength on the lysozyme adsorption. Moreover, the selectivity of surface imprinted cryogels was examined against cytochrome c and bovine serum albumin (BSA) as the competitors. Finally, the mathematical modeling, which was applied to describe the adsorption process, showed that the experimental data is very well-fitted to the Langmuir adsorption isotherm. © 2016 Elsevier B.V.
dc.identifier.citationErol, K., Köse, K., Uzun, L., Say, R., Denizli, A. (2016). Polyethyleneimine assisted-two-step polymerization to develop surface imprinted cryogels for lysozyme purification. Colloids and Surfaces B: Biointerfaces, 146, 567-576.
dc.identifier.doi10.1016/j.colsurfb.2016.06.060
dc.identifier.endpage576en_US
dc.identifier.issn0927-7765
dc.identifier.scopusqualityQ1
dc.identifier.startpage567en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2016.06.060
dc.identifier.urihttps://hdl.handle.net/11491/1079
dc.identifier.volume146en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofColloids and Surfaces B: Biointerfaces
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryogelen_US
dc.subjectLysozymeen_US
dc.subjectPolyethyleneimineen_US
dc.subjectSurface Imprintingen_US
dc.subjectTwo-Step Polymerizationen_US
dc.titlePolyethyleneimine assisted-two-step polymerization to develop surface imprinted cryogels for lysozyme purification
dc.typeArticle

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