Usnic acid causes apoptotic-like death in Leishmania major, L. infantum and L. tropica

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dc.authorid0000-0001-8421-3625
dc.contributor.authorDerici, Mehmet Kürşat
dc.contributor.authorCansaran Duman, Demet
dc.contributor.authorTaylan Özkan, Hikmet Ayşegül
dc.date.accessioned2019-05-13T09:08:51Z
dc.date.available2019-05-13T09:08:51Z
dc.date.issued2018
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractLeishmaniasis, a deadly parasitic infection, threatens many people worldwide. Since the high cost, toxicity, and resistance are drawbacks of current treatment options, it is necessary to find safer and more effective new antileishmanial drugs. The aim of this study was to determine the antileishmanial activity of usnic acid and its apoptotic mechanism on Leishmania spp. promastigotes. The antileishmanial activity was evaluated by MTT assay and apoptosis-related gene expression was investigated by qRT-PCR. Usnic acid was to be effective against Leishmania major, L. infantum, and L. tropica promastigotes at IC 50 = 10.76 µg/ml, 13.34 µg/ml, and 21.06 µg/ml, respectively. We also demonstrated a novel mechanism by which usnic acid inhibited proliferation and caused apoptosis; usnic acid upregulated p53, Bax, Casp-3, and Casp-9 gene expression and downregulated the level of Bcl-2 gene expression. Accordingly, the expression level of the P53 gene increased in L. major, L. infantum and L. tropica by 14.4-, 11.8-, and 9.5-fold, respectively, and in contrast, the Bcl-2 gene expression decreased in all three leishmaniasis by 0.8-, 0.8-, and 0.7-fold, respectively. The present study, therefore, revealed that usnic acid played a critical role in the usnic acid-induced apoptotic process in Leishmania species. Usnic acid is easily accessible and an inexpensive agent, and can be considered as an alternative therapeutic agent for Leishmania infections subject to further tests in animal models. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
dc.identifier.citationDerici, M. K., Cansaran Duman, D., Taylan-Özkan, A. (2018). Usnic acid causes apoptotic-like death in Leishmania major, L. infantum and L. tropica. 3 Biotech, 8(9), 384.
dc.identifier.doi10.1007/s13205-018-1409-6
dc.identifier.issn2190-572X
dc.identifier.issue9en_US
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s13205-018-1409-6
dc.identifier.urihttps://hdl.handle.net/11491/2063
dc.identifier.volume8en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Verlag
dc.relation.ispartof3 Biotech
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosisen_US
dc.subjectLeishmaniaen_US
dc.subjectLichenen_US
dc.subjectqRT-PCRen_US
dc.subjectUsnic Aciden_US
dc.titleUsnic acid causes apoptotic-like death in Leishmania major, L. infantum and L. tropica
dc.typeArticle

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