In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes
Yükleniyor...
Dosyalar
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
The Scientific and Technological Research Council of Turkey (TÜBİTAK)
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The organophosphate target enzyme paraoxonase-1 (PON1) has been extensively studied in toxicology. Pesticides are organophosphate compounds that are commonly used in agriculture. They are also used as nerve gases, such as sarin and soman. Therefore, the effects of these compounds on enzymatic activity are well known. In the present study, we investigated the in vitro effects of fenoxaprop-p-ethyl, lambda cyhalothrin, imidacloprid, and dichlorvos pesticides on sheep liver microsomal PON1. The enzyme was purified approximately 141-fold with a specific activity of 1822.22 EU/mg proteins. The pesticides inhibited sheep liver PON1 in vitro, and the IC_ {50} values for these compounds were 0.0103, 0.069, 0.157, and 0.2\mu M, respectively.
Açıklama
Anahtar Kelimeler
Paraoxonase, Fenoxaprop-p-ethyl, Lambda Cyhalothrin, Imidacloprid, Dichlorvos, Inhibition
Kaynak
Turkish Journal of Chemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
38
Sayı
3
Künye
Koncuk Cebeci, B., Alım, Z., Beydemir Ş. (2014). In vitro effects of pesticide exposure on the activity of the paraoxonase 1 enzyme from sheep liver microsomes. Turkish Journal of Chemistry, 38, 512-520.
