Detection and identification of cutaneous leishmaniasis isolates by culture, polymerase chain reaction and sequence analyses in Syrian and Central Anatolia patients
Yükleniyor...
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Saudi Arabian Armed Forces Hospital
Erişim Hakkı
Attribution-NonCommercial 4.0 International
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Objectives: To characterize the cutaneous leishmaniasis (CL) isolates of Syrian and Central Anatolia patients at species levels. Methods: Skin scrapings of 3 patients (2 Syrian, 1 Turkish) were taken and examined by direct examination, culture in Novy-MacNeal-Nicole (NNN) medium, Internal transcribed spacer Polymerase chain reaction and sequence analysis (PCR). Results: According to microscopic examination, culture and PCR methods, 3 samples were detected positive. The sequencing results of all isolates in the study were identified as L. tropica. The same genotypes were detected in the 3 isolates and nucleotide sequence submitted into GenBank with the accession number: KP689599. Conclusion: This finding could give information about the transmission of CL between Turkey and Syria. Because of the Syrian civil war, most of the Syrian citizens circulating in Turkey and different part of Europe, this can be increase the risk of spreading the disease. So, prevention measurements must be taken urgently. © 2017 Saudi Arabian Armed Forces Hospital. All rights reserved.
Açıklama
Anahtar Kelimeler
[Belirlenecek]
Kaynak
Saudi Medical Journal
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
38
Sayı
9
Künye
Beyhan, Y. E., Karakuş, M., Karagöz, A., Mungan, M., Taylan Özkan, H. A., Hökelek, M. (2017). Detection and identification of cutaneous leishmaniasis isolates by culture, Polymerase chain reaction and sequence analyses in syrian and central anatolia patients. Saudi Medical Journal, 37(9), 968-971.
