Detection and identification of cutaneous leishmaniasis isolates by culture, polymerase chain reaction and sequence analyses in Syrian and Central Anatolia patients

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dc.authorid0000-0001-8421-3625
dc.contributor.authorBeyhan, Yunus Emre
dc.contributor.authorKarakuş ,Mehmet
dc.contributor.authorKaragöz, Alper
dc.contributor.authorMungan, Mesut
dc.contributor.authorTaylan Özkan, Hikmet Ayşegül
dc.contributor.authorHökelek, Murat
dc.date.accessioned2019-05-13T08:57:28Z
dc.date.available2019-05-13T08:57:28Z
dc.date.issued2017
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractObjectives: To characterize the cutaneous leishmaniasis (CL) isolates of Syrian and Central Anatolia patients at species levels. Methods: Skin scrapings of 3 patients (2 Syrian, 1 Turkish) were taken and examined by direct examination, culture in Novy-MacNeal-Nicole (NNN) medium, Internal transcribed spacer Polymerase chain reaction and sequence analysis (PCR). Results: According to microscopic examination, culture and PCR methods, 3 samples were detected positive. The sequencing results of all isolates in the study were identified as L. tropica. The same genotypes were detected in the 3 isolates and nucleotide sequence submitted into GenBank with the accession number: KP689599. Conclusion: This finding could give information about the transmission of CL between Turkey and Syria. Because of the Syrian civil war, most of the Syrian citizens circulating in Turkey and different part of Europe, this can be increase the risk of spreading the disease. So, prevention measurements must be taken urgently. © 2017 Saudi Arabian Armed Forces Hospital. All rights reserved.
dc.identifier.citationBeyhan, Y. E., Karakuş, M., Karagöz, A., Mungan, M., Taylan Özkan, H. A., Hökelek, M. (2017). Detection and identification of cutaneous leishmaniasis isolates by culture, Polymerase chain reaction and sequence analyses in syrian and central anatolia patients. Saudi Medical Journal, 37(9), 968-971.
dc.identifier.doi10.15537/smj.2017.9.21025
dc.identifier.endpage971en_US
dc.identifier.issn0379-5284
dc.identifier.issue9en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage968en_US
dc.identifier.urihttps://doi.org/10.15537/smj.2017.9.21025
dc.identifier.urihttps://hdl.handle.net/11491/934
dc.identifier.volume38en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSaudi Arabian Armed Forces Hospital
dc.relation.ispartofSaudi Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/legalcode*
dc.subject[Belirlenecek]en_US
dc.titleDetection and identification of cutaneous leishmaniasis isolates by culture, polymerase chain reaction and sequence analyses in Syrian and Central Anatolia patients
dc.typeArticle

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