Serological screening of West Nile virus among blood donors in northern Cyprus

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dc.authorid0000-0001-9945-478X
dc.authorid0000-0001-8421-3625
dc.contributor.authorBalaman, Nagat
dc.contributor.authorGazi, Umut
dc.contributor.authorImir, Turgut
dc.contributor.authorŞanlıdağ, Tamer
dc.contributor.authorRuh, Emrah
dc.contributor.authorTosun, Özgür
dc.contributor.authorÖzgül, Aykut
dc.contributor.authorTaylan Özkan, Hikmet Ayşegül
dc.date.accessioned2020-02-14T11:07:51Z
dc.date.available2020-02-14T11:07:51Z
dc.date.issued2020en_US
dc.departmentHitit Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractBackground: West Nile virus (WNV) is a neurotropic arbovirus that can also be transmitted through blood transfusion. Even though its geographic distribution has been expanding, there has not yet been any epidemiological data on WNV in northern Cyprus. The aim of our study is to fill this gap by using donated blood samples. Methods: Samples collected from the main government hospital blood bank in Nicosia were analyzed by anti?WNV enzyme?linked immunosorbent assay (ELISA) (immunoglobulin M [IgM] and immunoglobulin G [IgG]). Seropositive samples were subjected to plaque reduction neutralization test (PRNT) for confirmation and analyzed by ELISA IgG avidity test and reverse transcription real?time polymerase chain reaction (rRT?PCR). Results: Of the 760 sera samples, 2 (0.3%) were IgM+ and 31 (4.1%) were IgG+. Neutralization activity was detected in none (0.0%) of the IgM+ and 26 (83.9%) of IgG+ donor specimens. ELISA IgG avidity test reported high avidity in 21 (67.7%) and low avidity in one (3.2%) IgG+ sample. PRNT?confirmed anti?WNV IgG+ samples exhibited only borderline (19.2%) or high avidity (80.8%) values. rRT?PCR results were negative for both IgM+ and IgG+ samples. Conclusion: Anti?WNV antibodies were detected in northern Cyprus among blood donors. The establishment of preventive measures and evaluation of the geographic extent of the WNV in northern Cyprus are highly recommended.
dc.description.provenanceSubmitted by Zeynep Umut Arslan (umutarslan@hitit.edu.tr) on 2020-02-14T11:07:31Z No. of bitstreams: 0en
dc.description.provenanceApproved for entry into archive by Zeynep Umut Arslan (umutarslan@hitit.edu.tr) on 2020-02-14T11:07:51Z (GMT) No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2020-02-14T11:07:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020en
dc.identifier.citationBalaman, N., Gazi, U., Imir, T., Sanlidag, T., Ruh, E., Tosun, O., Taylan?Ozkan, A. (2020). Serological Screening of West Nile Virus Among Blood Donors in Northern Cyprus. Journal of Medical Virology.
dc.identifier.doi10.1002/jmv.25669
dc.identifier.issn0146-6615
dc.identifier.issn1096-9071
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/jmv.25669
dc.identifier.urihttps://hdl.handle.net/11491/5741
dc.identifier.wosWOS:000510517700001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Medical Virology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAvidityen_US
dc.subjectELISAen_US
dc.subjectFlavivirusen_US
dc.subjectIgGen_US
dc.subjectIgMen_US
dc.subjectNorthern Cyprusen_US
dc.subjectReverse Transcription Real‐time PCRen_US
dc.titleSerological screening of West Nile virus among blood donors in northern Cyprus
dc.typeArticle

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