Selection of superior bifidobacteria in the presence of rotavirus
Yükleniyor...
Dosyalar
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Associacao Brasileira de Divulgacao Cientifica
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The main purpose of this study was to investigate bifidobacteria flora in fecal samples from children with rotavirus infection and determine the significance of their selected probiotic properties for improvement of health status. Enzyme-linked immunosorbent assay was used to identify rotavirus antigen in fecal samples from 94 patients with gastroenteritis and from 30 without gastroenteritis. Bifidobacteria were identified by selective media, gram reaction, colony morphology, fructose-6- phosphate phosphoketolase enzyme activity and classical identification tests. Exopolysaccharide (EPS) production was identified by phenol-sulphuric acid method. The modified method was then used to identify the quantity of taurocholic and glycocholic acid deconjugation and cholesterol elimination of the strains. Thirty-five of the 94 fecal samples were found positive for rotavirus antigen (37.23%). Bifidobacteria were identified in 59 of the samples. The EPS production ranges were 29.56- 102.21 mg/L. The cholesterol elimination rates ranged between 8.36-39.22%. Furthermore, a positive and strong correlation was determined between EPS production and the presence of cholesterol (r=0.984, P<0.001). The deconjugation rates for the sodium glycocholate group was higher than the sodium taurocholate group. Rotavirus (+) bifidobacteria strains had higher EPS production, deconjugation rate and cholesterol elimination compared to bifidobacteria strains isolated from children in the rotavirus (-) sample and without gastroenteritis. Significant differences were observed among groups in all parameters (P<0.05). Given the increased number of rotavirus cases in Turkey and worldwide, it is very important to add superior bifidobacteria in the diets of infected children to improve the intestinal and vital functions. © 2016, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.
Açıklama
Anahtar Kelimeler
Bifidobacteria, Bile Salt Deconjugation, Cholesterol Elimination, EPS Production, Rotavirus
Kaynak
Brazilian Journal of Medical and Biological Research
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
49
Sayı
11
Künye
Alp Avcı, G. (2016). Selection of superior bifidobacteria in the presence of rotavirus. Brazilian Journal of Medical and Biological Research, 49(11).
