PolyGuanine methacrylate cryogels for ribonucleic acid purification

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dc.authorid0000-0003-1467-2898
dc.contributor.authorKöse, Kazım
dc.contributor.authorUzun, Lokman
dc.date.accessioned2019-05-13T09:06:53Z
dc.date.available2019-05-13T09:06:53Z
dc.date.issued2016
dc.departmentRektörlük
dc.description.abstractThe isolation and purification of ribonucleic acid have attracted attention recently for the understanding of the functions in detail because of the necessity for the treatment of genetic diseases. In this study, guanine-incorporated polymeric cryogels were developed to obtain highly purified ribonucleic acid. The satisfactory purification performance was achieved with the guanine-incorporated poly (2-hydroxyethyl methacrylate-guanine methacrylate) cryogels. The most crucial advantages to use guanine as a functional monomer are to obtain a real natural interaction between guanine on the polymeric material and cytosine on the ribonucleic acid. Moreover, using cryogel with a highly porous structure and high swelling ratio provide advantages of getting more water within the structure to get more analyte to interact. The characterization of cryogels has proved the success of the synthesis and the perfect natural interaction to be taken place between the ligand (guanine methacrylate) and the cytosine in the ribonucleic acid molecules. Although the pores within the structure of cryogels are small, they provide efficient and fast adsorption. The chromatographic separation performance was investigated for different conditions (pH, temperature etc.). The desorption ratio and reusability were also analyzed at the end of the five adsorption-desorption cycles with no significant changes. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.identifier.citationKöse, K., Uzun, L. (2016). PolyGuanine methacrylate cryogels for ribonucleic acid purification. Journal of Separation Science, 39(10), 1998-2005.
dc.identifier.doi10.1002/jssc.201600199
dc.identifier.endpage2005en_US
dc.identifier.issn1615-9306
dc.identifier.issue10en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage1998en_US
dc.identifier.urihttps://doi.org/10.1002/jssc.201600199
dc.identifier.urihttps://hdl.handle.net/11491/1673
dc.identifier.volume39en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.relation.ispartofJournal of Separation Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryogelsen_US
dc.subjectGuanine Methacrylateen_US
dc.subjectNucleotidesen_US
dc.subjectPurificationen_US
dc.subjectRibonucleic Aciden_US
dc.titlePolyGuanine methacrylate cryogels for ribonucleic acid purification
dc.typeArticle

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