Synthesis and characterization of Ag+-decorated poly(glycidyl methacrylate) microparticle design for the adsorption of nucleic acids

Basit Öğe Kaydı
dc.authorid0000-0003-4767-6799
dc.authorid0000-0003-1467-2898
dc.contributor.authorErol, Kadir
dc.contributor.authorUzunoğlu, Aytekin
dc.contributor.authorKöse, Kazım
dc.contributor.authorSarıca, Büşra
dc.contributor.authorAvcı, Emre
dc.contributor.authorKöse, Dursun Ali
dc.date.accessioned2019-05-13T09:04:49Z
dc.date.available2019-05-13T09:04:49Z
dc.date.issued2018
dc.departmentHitit Üniversitesi, Alaca Avni Çelik Meslek Yüksekokulu, Mülkiyet Koruma ve Güvenlik Bölümü
dc.description.abstractIn this study, we report on the adsorption of RNA and DNA molecules by exploiting the high binding affinity of these nucleic acids to Ag+ ions anchored on magnetic poly(glycidyl methacrylate) (PGMA) microparticles. PGMA microparticles were synthesized and modified with nicotinamide which enabled to anchor Ag+ ions on the surface. The successful preparation of PGMA was confirmed by the presence of characteristic FTIR peaks. The ESR results showed that the incorporation of Fe–Ni salt to the polymeric structure provided a magnetic property to the microparticles. The amount of nicotinamide and Ag+ ions used to modify the surface of the particles were found to be 1.79 wt% and 52.6 mg Ag/g microparticle, respectively. The high affinity of nucleic acids to Ag+ ions were exploited for the adsorption studies. At the optimum working conditions, the adsorption capacity of microparticles was found to be 40.1 and 11.48 mg nucleic acid/g microparticle for RNA and DNA, respectively. Our study indicated that the use of novel Ag+-decorated magnetic PGMA particles can be successfully employed as adsorbents for fast, easy, and cost-friendly adsorption of nucleic acids with high purity as well as high in quantity. © 2018 Elsevier B.V.
dc.identifier.citationErol, K., Uzunoğlu, A., Köse, K., Sarıca, B., Avcı, E., Köse, D. A. (2018). Synthesis and characterization of Ag+-decorated poly (glycidyl methacrylate) microparticle design for the adsorption of nucleic acids. Journal of Chromatography B, 1081-1082, 1-7.
dc.identifier.doi10.1016/j.jchromb.2018.02.017
dc.identifier.endpage7en_US
dc.identifier.issn1570-0232
dc.identifier.scopusqualityQ2
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2018.02.017
dc.identifier.urihttps://hdl.handle.net/11491/1646
dc.identifier.volume1081-1082en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAdsorptionen_US
dc.subjectChromatographyen_US
dc.subjectDNAen_US
dc.subjectPoly(Glycidyl Methacrylate)en_US
dc.subjectRNAen_US
dc.subjectSilveren_US
dc.titleSynthesis and characterization of Ag+-decorated poly(glycidyl methacrylate) microparticle design for the adsorption of nucleic acids
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu