Fe(II)-Co(II) double salt incorporated magnetic hydrophobic microparticles for invertase adsorption

[ X ]

Tarih

2015

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Humana Press Inc.

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Invertase (?-fructofuranoside fructohydrolase, EC 3.2.1.26) is an enzyme widely used in the food industry. Its main function is the formation of glucose and fructose through hydrolysis of sucrose. For the separation and purification of this commercially important enzyme from aqueous solutions, magnetic poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), m-poly(HEMA-MATrp) microparticles were developed. Magnetic properties of microparticles are provided using ferromagnetic Fe(II)-Co(II) double salt. Characterization studies of magnetic microparticles were conducted via vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analysis. Specific surface area of magnetic microparticles is 6.75 m(2)/g. Because of all experiments performed in this study, the adsorption capability of magnetic microparticles was optimized by variation of different conditions (pH, interaction time, initial invertase concentration, temperature, and ionic strength) and maximum adsorption capacity was determined as 992.64 mg invertase/g magnetic microparticles.

Açıklama

Anahtar Kelimeler

Adsorption, Double Salt, Hydrophobic Microparticles, Invertase, Magnetic Separation

Kaynak

Applied Biochemistry and Biotechnology

WoS Q Değeri

N/A

Scopus Q Değeri

Q2

Cilt

177

Sayı

5

Künye

Köse, K., Erol, K., Emniyet, A. A., Köse, D. A., Alp Avcı, G., Uzun, L. (2015). Fe (II)-Co (II) double salt incorporated magnetic hydrophobic microparticles for Invertase adsorption. Applied Biochemistry and Biotechnology, 177(5), 1025-1039.