Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Ltd.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4 mg/g and 196.4 mg/g, respectively. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
Açıklama
Anahtar Kelimeler
Adsorption, Cryogel, Enzyme, Lipase, Membrane
Kaynak
Artificial Cells, Nanomedicine and Biotechnology
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
45
Sayı
3
Künye
Köse, K., Erol, K., Köse, D. A., Evci, E., Uzun, L. (2017). Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect. Artificial Cells, Nanomedicine and Biotechnology, 45(3), 574-583.
