Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect

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dc.authorid0000-0003-1467-2898
dc.authorid0000-0003-4767-6799
dc.contributor.authorKöse, Kazım
dc.contributor.authorErol, Kadir
dc.contributor.authorKöse, Dursun Ali
dc.contributor.authorEvci, Emre
dc.contributor.authorUzun, Lokman
dc.date.accessioned2019-05-13T09:07:42Z
dc.date.available2019-05-13T09:07:42Z
dc.date.issued2017
dc.departmentRektörlük
dc.departmentHitit Üniversitesi, Alaca Avni Çelik Meslek Yüksekokulu, Mülkiyet Koruma ve Güvenlik Bölümü
dc.departmentHitit Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractCryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4 mg/g and 196.4 mg/g, respectively. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.citationKöse, K., Erol, K., Köse, D. A., Evci, E., Uzun, L. (2017). Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect. Artificial Cells, Nanomedicine and Biotechnology, 45(3), 574-583.
dc.identifier.doi10.3109/21691401.2016.1161642
dc.identifier.endpage583en_US
dc.identifier.issn2169-1401
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage574en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2016.1161642
dc.identifier.urihttps://hdl.handle.net/11491/1857
dc.identifier.volume45en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofArtificial Cells, Nanomedicine and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAdsorptionen_US
dc.subjectCryogelen_US
dc.subjectEnzymeen_US
dc.subjectLipaseen_US
dc.subjectMembraneen_US
dc.titleAffinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect
dc.typeArticle

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