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Öğe Adsorption of Victoria Blue R (VBR) dye on magnetic microparticles containing Fe(II)–Co(II) double salt(Taylor and Francis Inc., 2016) Erol, Kadir; Köse, Kazım; Köse, Dursun Ali; Sızır, Ümit; Tosun Satır, İlknur; Uzun, LokmanMagnetic microparticles have many applications in various areas today. The aim of this study was to develop hydrophobic magnetic microparticles as an alternative to traditional methods for high capacity and low cost removal of Victoria Blue R (VBR), which is valuable for industry and commercial, from wastewater and to determine the VBR adsorption ability of this adsorbent. Fe(II)–Co(II) double salt incorporated magnetic poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan) [m-poly(HEMA-MATrp)] microparticles were synthesized and used as adsorbent. These microparticles were synthesized in aqueous dispersion medium via microemulsion polymerization using MATrp and HEMA monomers. Magnetic hydrophobic microparticles were characterized via Fourier transform infrared spectroscopy, scanning electron microscopy, and vibrating sample magnetometer. Adsorption experiments were conducted for different conditions (pH, interaction time, amount of microparticles, temperature, and ionic strength) in batch system. VBR adsorption capacity of magnetic hydrophobic microparticles was estimated as 89.46 ?mol/g. Adsorption–desorption cycles were repeated 4 times, and there was no significant decrease for the adsorption capacity observed. © 2015 Balaban Desalination Publications. All rights reserved.Öğe Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect(Taylor and Francis Ltd., 2017) Köse, Kazım; Erol, Kadir; Köse, Dursun Ali; Evci, Emre; Uzun, LokmanCryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4 mg/g and 196.4 mg/g, respectively. © 2016 Informa UK Limited, trading as Taylor & Francis Group.Öğe Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization(Walter De Gruyter Gmbh, 2021) Erol, Kadir; Tatar, Demet; Veyisoğlu, Aysel; Tokatlı, AliMicron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.Öğe Calcined Eggshell for the Removal of Victoria Blue R Dye from Wastewater Medium by Adsorption(2021) Tosun Satır, İlknur; Erol, KadirIn this study, the use of calcined eggshell (CE) as an adsorbent in removing Victoria Blue R (VBR) dyestuff from the solution medium was investigated. For this purpose, pH, interaction time, adsorbate concentration, amount of adsorbent, and salt effect parameters were studied to determine the appropriate adsorption conditions. The highest adsorption yield was obtained at pH 2, 2.0 g/L adsorbent, and a stirring time of 5 minutes. 97% of the dye was removed under optimum adsorption conditions. The results obtained from the experimental studies showed that the adsorption mechanism is compatible with the pseudo-second-order kinetic model and the Langmuir isotherm model. SEM and IR analysis were performed for the characterization of CE.Öğe Development of novel copper modified cryogels for biochemical isolation(Springer, 2020) Köse, Kazım; Erol, Kadir; Köse, Dursun AliCu(II) anchored polymeric cryogels are synthesized for the isolation of B12. The poly(2-hydroxyethyl methacrylate) [poly(HEMA)] is used as solid support and N-methacryloyl-l-aspartic acid, is used as a ligand. Synthesis success is proved by the Fourier transform infrared spectroscopy, H-1 and C-13 nuclear magnetic resonance, scanning electron microscope, N-2 adsorption method (Brunauer, Emmet ve Teller), elemental analysis, induction coupled plasma optical emission spectroscopy. In the first part of the work, the polymeric material is synthesized and characterized, and in the second part, adsorptive treatment was carried out to determine the optimum B12 adsorption conditions with this synthesized material in varying conditions. The B12 isolation from cheese is carried out to determine the performance of the polymeric material in the real environment. The antioxidant capacity of the obtained B12 is an indication that the isolation process is successfully carried out.Öğe DNA adsorption via Co(II) immobilized cryogels(Taylor and Francis Inc., 2016) Erol, KadirThe separation and purification of important biomolecule deoxyribonucleic acid (DNA) molecules are extremely important. The adsorption technique among these methods is highly preferred as the adsorbent cryogels are pretty much used due to large pores and the associated flow channels. In this study, the adsorption of DNA via Co(II) immobilized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [poly(HEMA-GMA)] cryogels was performed under varying conditions of pH, interaction time, initial DNA concentration, temperature, and ionic strength. For the characterization of cryogels; swelling test, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), surface area (BET), elemental and ICP-OES analysis were performed. L-lysine amino acid was chosen as Co(II)-chelating agent and the adsorption capacity of cryogels was determined as 33.81 mg DNA/g cryogel. Adsorption of pea DNA was studied under the optimum adsorption conditions and DNA adsorption capacity of cryogels was found as 10.14 mg DNA/g cryogel. The adsorption process was examined via Langmuir and Freundlich isotherm models and the Langmuir adsorption model was determined to be more appropriate for the DNA adsorption onto cryogels. © 2016 Taylor & Francis Group, LLC.Öğe Effect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels(Elsevier B.V., 2019) Erol, Kadir; Koncuk Cebeci, Büşra; Köse, Kazım; Köse, Dursun AliHydrogen peroxide is converted by catalase to molecular oxygen and water to remove oxidative stress. In this study, catalase immobilization was performed using poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) (poly(HEMA-GMA)) cryogels with different amounts of GMA. Catalase adsorption capacity of 298.7 ± 9.9 mg/g was achieved at the end of 9 h using the poly(HEMA-GMA)-250 cryogel. Kinetic parameters and the inhibitory effects of pesticides such as 4,4?-DDE and 4,4?-DDT on the activity of free and immobilized catalase enzyme were investigated. While the V max value of the immobilized enzyme was reduced 4-fold compared to the free enzyme, in the case of the comparison of the K M values, the affinity of the immobilized enzyme was increased by 1.94 times against the substrate. The inhibitory effect of 4,4?-DDT pesticide was found to be higher for the immobilized and free enzyme. NaCl (1 M, pH: 7.0) solution was used for desorption of the adsorbed catalase enzyme. A desorption ratio of 96.45% was achieved. The technique used in this study is promising regarding for the immobilization of catalase enzyme to increase the operational activity. Therefore, poly(HEMA-GMA) cryogels have the potential to be used for immobilization of catalase enzyme in the fields of biology and biochemistry. © 2018 Elsevier B.V.Öğe Efficient polymeric material for separation of human hemoglobin(Taylor and Francis Ltd., 2017) Erol, Kadir; Köse, KazımIn this study, negatively charged acrylic acid was used as a functional ligand to synthesise net-poly(2-hydroxyethyl methacrylate-co-acrylic acid) microparticles for the removal of hemoglobin having a positively charged Fe2+?ion in the core, as an alternative to conventional techniques to achieve a cost effective high-capacity purification. The characterization of microparticles was performed via Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, atomic force microscopy, the Brunauer – Emmett – Teller surface area analysis, and swelling test methods. Optimum adsorption conditions such as pH, initial hemoglobin concentration, temperature, and interaction time were studied batch wise. The highest adsorption capacity of microparticles was observed at pH 7.4 as 1276.4?mg hemoglobin/g polymer. The reusability of microparticles was also promising with ?5% decrease in the adsorption capacity at the end of the five adsorption-desorption cycles.Öğe Electrostatic adsorption of asymmetric dimethyl arginine (adma) on poly (2-hydroxyethyl methacrylate-acrylic acid) nanoparticles(Taylor and Francis Inc., 2017) Erol, Kadir; Köse, Kazım; Avcı, Emre; Köse, Dursun AliSeparation and purification of asymmetric dimethylarginine (ADMA) molecule, which is an important biomolecule in terms of cardiovascular diseases, is of great importance. Among the methods, the adsorption technique is of considerable demand, and as an adsorbent, the nanoparticles are widely used. In this study, an ADMA isolation was performed via a novel method. Therefore, ADMA adsorption was achieved using a poly (2-hydroxyethyl methacrylate-acrylic acid) (poly(HEMA-AA)) nanoparticles. The change in the adsorption capacity was investigated in terms of changing interaction time, initial ADMA concentration, stirring rate, temperature and ionic strength. The functional group on the polymeric nanoparticles was characterized using Fourier Transform Infrared Spectroscopy (FT-IR); the surface morphology was determined via scanning electron microscopy (SEM), transient electron microscopy (TEM) and surface area (BET) analyzes. The Elisa Spectrophotometer was used for the quantitative analysis of the ADMA molecule. The adsorption capacity of the nanoparticles was determined as 23.76 mg/g. The adsorption process was characterized according to the isotherm calculation. © 2017 Taylor & Francis Group, LLC.Öğe Fe(II)-Co(II) double salt incorporated magnetic hydrophobic microparticles for invertase adsorption(Humana Press Inc., 2015) Köse, Kazım; Erol, Kadir; Emniyet Sert, Asiye Aslı; Köse, Dursun Ali; Alp Avcı, Gülçin; Uzun, LokmanInvertase (?-fructofuranoside fructohydrolase, EC 3.2.1.26) is an enzyme widely used in the food industry. Its main function is the formation of glucose and fructose through hydrolysis of sucrose. For the separation and purification of this commercially important enzyme from aqueous solutions, magnetic poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), m-poly(HEMA-MATrp) microparticles were developed. Magnetic properties of microparticles are provided using ferromagnetic Fe(II)-Co(II) double salt. Characterization studies of magnetic microparticles were conducted via vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analysis. Specific surface area of magnetic microparticles is 6.75 m(2)/g. Because of all experiments performed in this study, the adsorption capability of magnetic microparticles was optimized by variation of different conditions (pH, interaction time, initial invertase concentration, temperature, and ionic strength) and maximum adsorption capacity was determined as 992.64 mg invertase/g magnetic microparticles.Öğe Investigation of immobilization and kinetic properties of catalase enzyme on poly(HEMA-GMA) cryogel(Slovak Academy of Sciences Institute of Experimental Physics Department of Biophysics, 2017) Erol, Kadir; Cebeci, Büşra; Köse, Kazım; Köse, Dursun AliCatalase is a metalloenzyme, which is found in almost all aerobic respiratory organisms. This enzyme acts as a detoxification agent for the hydrogen peroxide (H2O2) compound, protecting tissues and cells from oxidative damage [1]. Cryogels are synthetic materials commonly used in adsorption and immobilization studies in recent years. In this study, it is aimed to investigate immobilization conditions of catalase enzyme poly(HEMA-GMA) cryogel for 3, 6, 9, 12, 18 and 24 hours in stationary solution and to determine how the activity of immobilized catalase enzyme will be changed by pesticides. This is very important in determining the effect of antioxidant activity and catalase, which is an important enzyme in organisms, to environmental pollution. For the characterization of cryogels; swelling test, Fourier transform infrared spectroscopy (FT-IR) analysis, scanning electron microscopy (SEM) analysis and elemental analysis.Öğe Isolation of aspartic acid using Novel Poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-lysine) Cryogels(Friedr. Vieweg und Sohn Verlags GmbH, 2018) Sarıca, Büşra; Köse, Kazım; Uzunoğlu, Aytekin; Erol, Kadir; Köse, Dursun AliIn this study, we report on the synthesis of novel poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-lysine) [poly(HEMA-MAL)] cryogels for the isolation of aspartic acid. Cryogels were characterized using nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), N2-adsorption, swelling test, and elemental analysis techniques. The FTIR and elemental analysis results showed successful synthesis of poly(HEMA-MAL)-based polymeric structures. SEM images and N2 adsorption results indicated the three-dimensional morphology and large surface area of the cryogels. After the synthesis and characterization steps, adsorption performance of these novel cryogels towards l-aspartic acid was evaluated and adsorption capacity was found to be 265.7 ± 3 mg aspartic acid g?1 adsorbent. To show the applicability of our adsorbent design for the isolation of aspartic acid from real samples, adsorption and desorption experiments using lysozyme enzyme were also performed. Our results showed that the proposed cryogel design can be successfully employed for the isolation of amino acids from complex environments. It can be stated that poly(HEMA-MAL)-based cryogels are promising candidates for the isolation of aspartic acid and lysozyme owing to their enhanced performance and specificity, low cost, and high reusability. © 2017, Springer-Verlag GmbH Germany.Öğe Lead removal from industrial waste(Hitit University, 2017) Bilgin, Elif; Yüce, Murat; Köse, Kazım; Erol, Kadir; Köse, Dursun AliIn this study, the heavy metal removal performance of poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester), poly(HEMA-MAH) cryogel, a known polymer, in HNO3 was investigated. To determine the heavy metal removal performance of cryogel, wastewater was used as obtained from an inorganic material manufacturer. The characterization studies using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, elemental analysis, waterswelling tests and surface area analysis were conducted. The effect of HNO3 on the adsorption process, especially for metals, was found very effective even in trace amounts.Öğe Magnetic diatomite for pesticide removal from aqueous solution via hydrophobic interactions(Springer Heidelberg, 2019) Erol, Kadir; Yildiz, Emrecan; Alacabey, Ihsan; Karabork, Muharrem; Uzun, LokmanPesticides are highly hazardous chemicals for the environment and human health and their use in agriculture is constantly increasing. Although 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane 4,4'-DDT was banned at developed countries, it is still one of the most dangerous of chemical due to accumulation in the environment. It is known that the toxicity of DDT affects some enzyme systems biochemically. The main motivation of this study is to develop an effective adsorbate for the removal DDT, which was chosen as a model hydrophobic pesticide, out of aqueous systems. For this purpose, the bare diatomite particles were magnetically modified and a hydrophobic ligand attached to enhance its adsorptive and physio-chemical features. Under optimal conditions, a high adsorption capacity, around 120 mg/g with the hydrophobic and magnetic diatomite particles, modification of the diatomite particles reduced average pores diameter whereas surface area and total pore volume increased (around 15-folds). After five consecutive adsorption-desorption cycles, no significant decrease in adsorption capability was observed. The adsorption isotherms (Langmuir, Freundlich, and Flory-Huggins) applied to the data indicated that the adsorption process occurred via monolayer adsorption in an entropy-driven manner. The kinetic data also revealed the quick adsorption process without any diffusion limitations.Öğe Nicotinamide-modified poly(HEMA-GMA)-Nic cryogels for removal of pesticides(Turkish Chemical Society, 2018) Köse, Kazım; Arslan Akveran, Gönül; Erol, Kadir; Köse, Dursun AliChlordane is only one of the persistent pesticides used in some countries despite the ban. Removal of chlordane, a severe threat to all living things, was performed using nicotinamide-modified poly (2-hydroxyethyl methacrylate-glycidyl methacrylate), poly(HEMA-GMA)-Nic, polymeric cryogels in this study. Poly(HEMA-GMA) polymeric cryogels were synthesized based on previously reported literature procedures and were subsequently modified by nicotinamide moieties. Removal of chlordane in alcoholic medium has been accomplished exploiting the alcoho-phobic interaction, which was the first indication in our previous study. Structural analysis of poly(HEMA-GMA)-Nic was performed using Fourier transform infrared spectroscopy (FT-IR) and elemental analysis methods. Scanning electron microscopy (SEM) was used to understand the surface morphology of cryogels. Surface area and cavity volume calculations were determined by applying N 2 adsorption method and swelling test. The interaction time and maximum adsorption capacity were identified as 5 minutes and 64.61 mg chlordane/g cryogel for 300 mg/L chlordane concentration and 108.818 mg chlordane/g cryogel for 800 mg/L chlordane concentration during the adsorption experiments. Cyclohexane, toluene, chloroform, dichloromethane, acetone, and acetonitrile were used as solvents to observe the solvent effect on adsorption of chlordane onto the polymeric material. As expected, the removal of chlordane was performed with the highest adsorption performance in cyclohexane with the lowest dielectric constant. © 2018, Turkish Chemical Society. All rights reserved.Öğe Pesticide effect on kinetic properties of catalase enzyme(Hitit Üniversitesi, 2017) Erol, Kadir; Cebeci, Büşra; Köse, Kazım; Köse, Dursun AliCatalase is a metalloenzyme. Contains Cu(II) as the metal ion. It is a detoxification agent for the H2O2 compound and protects tissues and cells from oxidative damage [1]. Cryogels are synthetic materials generally used in adsorption and immobilization studies of late years. In this study, it is aimed to investigate how the activity of free and immobilized catalase enzyme will be changed by pesticides (DDE and DDT). This is very important to determine how enzyme activity is affected by environmental pollution. For the characterization of cryogels; swelling test, Fourier transform infrared spectroscopy (FT-IR) analysis, scanning electron microscopy (SEM) analysis.Öğe Poly(HEMA-co-AA) microparticles for removal of aluminum: The reason for Alzheimer's(Taylor and Francis Inc., 2017) Yüce, Murat; Bilgin, Elif; Köse, Kazım; Erol, Kadir; Köse, Dursun AliAluminum is one of the most toxic metals causing a variety of neurologic diseases, especially Alzheimer's disease. It is impossible to avoid contact with aluminum because of its existence in food to medications. Therefore, removal of aluminum from the blood or wastewater is urgently important. The cost-effective and easy-to-prepare adsorbents are needed to get efficient aluminum removal. For that purpose, the poly(2-hydroxyethylmethacrylate-co-acrylic acid), poly(HEMA-co-AA), microparticles was synthesized to remove aluminum in a very short interaction time. The achievement of the desired polymeric structure was confirmed via Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and transmission electron microscope (TEM), etc. Additionally, particle features such as swelling ratio, size, and surface area were determined. The microparticles synthesized in this study have been determined with very good adsorption capacity even in small aluminum concentrations.Öğe PolyAdenine cryogels for fast and effective RNA purification(Elsevier B.V., 2016) Köse, Kazım; Erol, Kadir; Özgür, Erdoğan; Uzun, Lokman; Denizli, AdilCryogels are used effectively for many diverse applications in a variety of fields. The isolation or purification of RNA, one of the potential utilizations for cryogels, is crucial due to their vital roles such as encoding, decoding, transcription and translation, and gene expression. RNA principally exists within every living thing, but their tendency to denaturation easily is still the most challenging issue. Herein, we aimed to develop adenine incorporated polymeric cryogels as an alternative sorbent for cost-friendly and fast RNA purification with high capacity. For this goal, we synthesized the polymerizable derivative of adenine called as adenine methacrylate (AdeM) through the substitution reaction between adenine and methacryloyl chloride. Then, 2-hydroxyethyl methacrylate (HEMA)-based cryogels were prepared in a partially frozen aqueous medium by copolymerization of monomers, AdeM, and HEMA. The cryogels were characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), surface area measurements, thermogravimetric analysis (TGA), and swelling tests. RNA adsorption experiments were performed via batch system while varying different conditions including pH, initial RNA concentration, temperature, and interaction time. We achieved high RNA adsorption capacity of cryogels, with the swelling ratio around 510%, as 11.86 mg/g. The cryogels might be reused at least five times without significant decrease in adsorption capacity. © 2016 Elsevier B.V.Öğe Polychelated cryogels: hemoglobin adsorption from human blood(Taylor and Francis Ltd., 2017) Erol, KadirThe separation and purification methods are extremely important for the hemoglobin (Hb) which is a crucial biomolecule. The adsorption technique is popular among these methods and the cryogels have been used quite much due to their macropores and interconnected flow channels. In this study, the Hb adsorption onto the Cu(II) immobilized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), poly(HEMA-GMA)-Cu(II), cryogels was investigated under different conditions (pH, interaction time, initial Hb concentration, temperature and ionic strength) to optimize adsorption conditions. The swelling test, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), surface area (BET), elemental and ICP-OES analysis were performed for the characterization of cryogels. Polyethyleneimine (PEI) molecule was used as a Cu(II)-chelating ligand. The Hb adsorption capacity of cryogels was determined as 193.8 mg Hb/g cryogel. The isolation of Hb from human blood was also studied under optimum adsorption conditions determined and the Hb (124.5 mg/g cryogel) was isolated. The adsorption model was investigated in the light of Langmuir and Freundlich adsorption isotherm models and it was determined to be more appropriate to the Langmuir adsorption isotherm model. © 2016 Informa UK Limited, trading as Taylor & Francis Group.Öğe Polyethyleneimine assisted-two-step polymerization to develop surface imprinted cryogels for lysozyme purification(Elsevier B.V., 2016) Erol, Kadir; Köse, Kazım; Uzun, Lokman; Say, Rıdvan; Denizli, AdilSurface imprinting strategy is one of the promising approaches to synthesize plastic antibodies while overcoming the problems in the protein imprinting research. In this study, we focused our attentions on developing two-step polymerization to imprint on the bare surface employing polyethyleneimine (PEI) assisted-coordination of template molecules, lysozyme. For this aim, we firstly synthesized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), poly(HEMA-GMA) cryogels as a bare structure. Then, we immobilized PEI onto the cryogels through the addition reaction between GMA and PEI molecules. After that, we determined the amount of free amine (NH2) groups of PEI molecules, subsequently immobilized methacrylate functionalities onto the half of them and another half was used to chelate Cu(II) ions as a mediator between template, lysozyme and PEI groups. After the characterization of the materials developed by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and the micro-computed tomography (?CT), we optimized the lysozyme adsorption conditions from aqueous solution. Before performing lysozyme purification from chicken egg white, we evaluated the effects of pH, interaction time, the initial lysozyme concentration, temperature and ionic strength on the lysozyme adsorption. Moreover, the selectivity of surface imprinted cryogels was examined against cytochrome c and bovine serum albumin (BSA) as the competitors. Finally, the mathematical modeling, which was applied to describe the adsorption process, showed that the experimental data is very well-fitted to the Langmuir adsorption isotherm. © 2016 Elsevier B.V.
